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Tetraphenylphosphonium Bromide (TPP) Large Molecule~Small Molecule Interactions

Tetraphenylphosphonium Bromide (TPP) Large Molecule~Small Molecule Interactions . Pilgrim High School Mr. Dubois, Mr. Lobdell C.J. Beneduce, Bryan Hallinan, Amanda Legare, Ed White. Chemical Compound. TPP: (C 6 H 5 ) 4 P(Br) Molecular Weight: 419.31 AMU 2.86E-10 mole TPP/ μ l d. H20

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Tetraphenylphosphonium Bromide (TPP) Large Molecule~Small Molecule Interactions

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  1. Tetraphenylphosphonium Bromide (TPP)Large Molecule~Small Molecule Interactions Pilgrim High School Mr. Dubois, Mr. Lobdell C.J. Beneduce, Bryan Hallinan, Amanda Legare, Ed White

  2. Chemical Compound • TPP: (C6H5)4P(Br) • Molecular Weight: 419.31 AMU • 2.86E-10 mole TPP/μl d. H20 • Melting Point Range: 295 - 299°C • Soluble in water • Other physical properties and toxicity levels not available • Hazardous Decomposition Products: Carbon monoxide, carbon dioxide, hydrogen bromide gas, thermal decomposition may produce toxic fumes of phosphorus oxides and/or phosphine.

  3. Genomic DNA Melting

  4. Genomic DNA Melting

  5. ELECTROPHORESIS Ladder According to the electrophoresis test, it appears that the cut plasmid has 4,000 bases. This supports that the plasmid had no significant effect on the DNA. *33nanoGrams (nG) (cf. Biolabs bulletin www.neb.com/nebecomm/products3232.asp)

  6. HyperChem Predictions

  7. HyperChem Predictions • For all optimization Polak Ribiere was used • Both AMBER and MM+ were used

  8. Discussion • HyperChem analyses led to the prediction that Tetraphenylphosphonium Bromide would bond with DNA. The experimental results contrasted with this prediction. According to the results of the electrophoresis and the melting curve, TPP did not have an impact on the DNA molecule. • Dr. Williams et al performed related experiments, including electrophoresis, and also found that TPP does not bond with DNA. • Other compounds will be studied throughout the school year. A compound that interacts with DNA is hoped to be found using similar experiments.

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