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Gel Templates

The following slides can be used as templates in mocking up your PCR Gels. Open up an image of your gel. Copy/Paste or import your gel figure onto the slide using Picture option in the Powerpoint Insert pull-down menu.

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Gel Templates

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  1. The following slides can be used as templates in mocking up your PCR Gels. • Open up an image of your gel. • Copy/Paste or import your gel figure onto the slide using Picture option in the Powerpoint Insert pull-down menu. • Size the gel to roughly fit the page and the lane markers. Note: If your gel has different dimensions or number of lanes then you will not be able to make them line up well and will need to adjust the labels. • Copy, move and resize the labels and size markers as necessary • Replace the #XX#.10 labels with your intitials and year(e.g. MS.11). • Indicate the predicted size of the cDNA inserts at the bottom of the gel (###). • Discuss the size of your cDNA insert (subtract 200 bp’s to consider vector bp’s). • State if it should be sent out for sequencing (>300bp’s) bp= nucleotide base pairs. • Save the file and upload it to your school’s P drive. • Prepare a PowerPoint presentation for your PCR gel including the size of the cDNA and whether it would be sent out for sequencing as mentioaed in #7 and 8. Gel Templates

  2. PCR gel template: SAMPLE Put your name here Put your initial by the insert that is yours 57PCRG A or B.11 M 2MS 2XX 4XX 5XX 6XX 7XX 4000 3000 2000 1650 1000 850 650 500 400 300 200 100 Insert size ### ### ### ### ### ### ### ### ###

  3. Period 6 Put your name here Put your initial by the insert that is yours 57PCRG3.11 A M 2XX 3XX 4XX 5XX 6XX eXX 7XX 4000 3000 2000 1650 1000 850 650 500 400 300 200 100 Insert size ## ## ## ## ## ## ##

  4. Period 6 Put your name here Put your initial by the insert that is yours 57PCRG.11 B M 3XX M 2XX 4XX 5XX 6XX 7XX 4000 3000 2000 1650 1000 850 650 500 400 300 200 100 Insert size ## ## ## ## ## ## ##

  5. PCR gel template: Extra – Get your number from Mrs. Sheldon Put your name here Put your initial by the insert that is yours 57PCRG A or B.11 M 2MS 2XX 4XX 5XX 6XX 7XX 9XX 8XX 4000 3000 M M 2000 1650 1000 850 650 500 400 300 200 100 Insert size ### ### ### ### ### ### ### ### ###

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