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ICEmB

ICEmB. THz exposure of whole blood for the study of biological effects on human lymphocytes M. R. Scarfì, M. Romanò, R. Di Pietro, O. Zeni CNR-IREA Napoli (I) A.Doria, G. P. Gallerano, E. Giovenale, G. Messina, A. Lai, G. Campurra, D. Coniglio, M. D’Arienzo ENEA – FIS-ACC Frascati (I).

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ICEmB

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  1. ICEmB THz exposure of whole blood for the study of biological effects on human lymphocytes M. R. Scarfì, M. Romanò, R. Di Pietro, O. Zeni CNR-IREA Napoli (I) A.Doria, G. P. Gallerano, E. Giovenale, G. Messina, A. Lai, G. Campurra, D. Coniglio, M. D’Arienzo ENEA – FIS-ACC Frascati (I) Capri, September 29 - October 2 2002

  2. ICEmB To study the potential health hazard of THz radiation since very little is known about its interaction with biological systems Specialised personnel and users in the laboratory-scale THz sources in use at research labs technical personnel and future patient exposure due to recent technological applications of THz radiation in biology and medicine, based on the specific spectroscopic fingerprints of biological matter in this spectral region Capri, September 29 - October 2 2002

  3. ICEmB Genotoxic effects a close correlation exists between DNA damage and cancer occurence Human peripheral blood lymphocytes well known biological model Cytogenetic techniques cytokinesis block MN assay is a very sensitive and simple indicator of chromosome damage, which also provides information on cell cycle progression AIM: to study MN induction in HPBL exposed in G0 phase to 120 and 130 GHz THz radiation Capri, September 29 - October 2, 2002

  4. a b c d ICEmB THz Irradiation Compact FEL Facility at the ENEA Research Center a = light pipe b = TDS c = right-angle bend d = pyroelectric detector Capri, September 29 - October 2, 2002

  5. ICEmB Absorption measurements on whole blood at 120 GHz Optimal thickness of the sample:1 mm Optimal irradiation geometry to contain a minimum useful whole blood volume of 2 ml:Petri dishes with internal diameter of 52 mm Capri, September 29 - October 2, 2002

  6. ICEmB Irradiation Parameters Capri, September 29 - October 2, 2002

  7. ICEmB Blood collection at the ENEA occupational health unit by venipuncture in heparinized vacutainers Donors 9 healthy subjects aged between 30 and 45 years (mean age: 39.33 ± 4.82 y) neither therapeutic drugs nor diagnostic X rays Capri, September 29 - October 2, 2002

  8. ICEmB • Biological procedure • for each donor, 3 conditions: • exposed • sham exposed • control 2 ml wb in 52 mm Petri dishes Sham exposure rationale: to simulate the same environmental conditions of exposed samples, but in absence of radiation Capri, September 29 - October 2, 2002

  9. ICEmB Lymphocyte cultures Following 20 min. exposure/sham exposure, 0.8 ml wb in 9.2 ml RPMI 1640 medium containing 15% FCS, 2 mM L-Glutamine and 100 µl PHA control cultures handled in parallel Capri, September 29 - October 2, 2002

  10. ICEmB Cytokinesis-block micronucleus assay Screening MN frequency: % CB cells containing MN/1000 CB cells CBPI: by classifying 500 cells according to the n° of nuclei [M1 + 2M2 + 3(M3+M4)/N] Statistical analysis two tailed paired Student’s t test Capri, September 29 - October 2, 2002

  11. ICEmB 1000X Capri, September 29 - October 2, 2002

  12. ICEmB MN frequency in HPBL cultures following THz radiation exposure in G0 phase (9 subjects) at 120 and 130 GHz 120 GHz – 6 donors 130 GHz – 3 donors P>0.05 Capri, September 29 - October 2, 2002

  13. ICEmB CBPI in HPBL cultures following THz radiation exposure in G0 phase (9 subjects) at 120 and 130 GHz P>0.05 Capri, September 29 - October 2, 2002

  14. ICEmB Conclusions • Environmental condition at FEL laboratory does not influence MN induction and cell cycle kinetics of HPBL (sham/control); • THz exposure of HPBL, at both 120 and 130 GHz: • does not affect MN formation; • does not influence cell proliferation (sham/exposed). • Ongoing studies on a larger number of individuals should provide further, more complete information on this topic. Capri, September 29 - October 2, 2002

  15. ICEmB • Future work • exposed/sham exposed • different exposure conditions: • f (following input from WP1); power levels • modulation conditions: still open • possibility of extending the irradiation measurements to the range 1-10 THz at other partners sites Capri, September 29 - October 2, 2002

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