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Biological Agent Identification Technologies. Dr. Mildred Donlon. Biological Role of Channels. Single Channel Currents. Patch Pipette. Bilayer Set Up and Boundary Conditions. Side 2: Insulator. Ion. Ionic Channel. End 1: Electrode. Ion. End 2: Electrode. Ion. Ion. Ion.
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Biological Agent Identification Technologies Dr. Mildred Donlon
Biological Role of Channels Dr. Donlon: Biological Agent Identification Technologies
Single Channel Currents Dr. Donlon: Biological Agent Identification Technologies
Patch Pipette Dr. Donlon: Biological Agent Identification Technologies
Bilayer Set Up and Boundary Conditions Side 2: Insulator Ion Ionic Channel End 1: Electrode Ion End 2: Electrode Ion Ion Ion Ion Ion Ion Side 1: Insulator Membrane Ion Dr. Donlon: Biological Agent Identification Technologies
Ion Channel Protein Ion Ion Ion Ion Ion Ion Channel’s Pore Ion Ion Channel Protein Ions in Channel Protein Dr. Donlon: Biological Agent Identification Technologies
Channels have Inputs and Outputs Channels have Complex Structure that allows Simple Function just like amplifiers! Channels follow simple rules, just like amplifiers Channels as Devices Dr. Donlon: Biological Agent Identification Technologies
Trial and Error or Theory ?? Why Theory? You Choose ! Dr. Donlon: Biological Agent Identification Technologies
Crowded Charge What Theory? Dr. Donlon: Biological Agent Identification Technologies
Selectivity Dr. Donlon: Biological Agent Identification Technologies
It does not matter DFT (Density Functional) Nonner, Gillespie & Eisenberg MC (Monte Carlo) Henderson, et al MSA (Mean Spherical Approximation) Nonner & Eisenberg MCV (Monte Carlo Variational) Hansen et al All give the same results What Version of Theory? Dr. Donlon: Biological Agent Identification Technologies
Make a Calcium Channel ? Dr. Donlon: Biological Agent Identification Technologies
Make a Calcium Channel ?? We are trying !! Dr. Donlon: Biological Agent Identification Technologies
Cl− Channel Dr. Donlon: Biological Agent Identification Technologies
Chloride Channel Selectivity Dr. Donlon: Biological Agent Identification Technologies
Conclusions • Crowded Charge can account for Selectivity • Crowded Charge is important for all Protein Function. Dr. Donlon: Biological Agent Identification Technologies
Future Work Prove it ! Dr. Donlon: Biological Agent Identification Technologies
Many thanks … For a job well done!! To Millie Donlon Dr. Donlon: Biological Agent Identification Technologies