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ELUTION DIAGRAM OF CYTOCHROME-C FROM ION-EXCHANGE MAGNETIC NANOPARTICLES

ELUTION DIAGRAM OF CYTOCHROME-C FROM ION-EXCHANGE MAGNETIC NANOPARTICLES. FERMENTATION PROFILE OF Pichia pastoris : PRODUCTION OF RECOMBINANT DROSOMYCIN. ADSORPTION ISOTHERM OF DROSOMYCIN FERMENTATION BROTH ON MAGNETIC NANOPARTICLES. SUMMARY OF DROSOMYCIN PURIFICATION USING

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ELUTION DIAGRAM OF CYTOCHROME-C FROM ION-EXCHANGE MAGNETIC NANOPARTICLES

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  1. ELUTION DIAGRAM OF CYTOCHROME-C FROM ION-EXCHANGE MAGNETIC NANOPARTICLES

  2. FERMENTATION PROFILE OF Pichia pastoris: PRODUCTION OF RECOMBINANT DROSOMYCIN

  3. ADSORPTION ISOTHERM OF DROSOMYCIN FERMENTATION BROTH ON MAGNETIC NANOPARTICLES

  4. SUMMARY OF DROSOMYCIN PURIFICATION USING MAGNETIC NANOPARTICLES

  5. SDS GEL ELECTROPHORESIS USING MAGNETIC NANOPARATICLES: ELUTION PROFILES OF DROSOMYCIN

  6. COMPARISON OF MAGNETIC NANOPARTICLES WITH OTHER PURIFICATION SCHEMES

  7. NEW CONCEPT IN INCREASING OXYGEN TRANSFER RATE USING MAGNETIC NANOPARTICLES Magnetic particle Oleic Acid 20 nm 30-50 nm • Low amount of coating • Very high interfacial areas • Readily recovered by magnetic filtration

  8. Aqueous solution of FeCl2 and FeCl3 NH4OH 80ºC Oleic acid coating Hitenol coating SYNTHESIS OF MAGNETIC NANO-PARTICLES • Fast (30 min) simple synthesis (stirred tank) • Inexpensive, readily available materials

  9. MASS TRANSFER CHARACTERIZATION IN BIOREACTORS: SULFITE OXIDATION air to mass spec Di = 10cm HL = 14.5cm [SO32-] = 0.67M [Cu2+] = 1x10-3 M VTOTAL = 20L VWORKING = 5.5L Dtank = 22cm

  10. OXYGEN MASS TRANSFER COEFFICIENT VERSUS GASSED POWER PER UNIT VOLUME IN 20-LITER BIOREACTOR Ф: particle wt%

  11. OXYGEN MASS TRANSFER IN E. coli FERMENTATIONS • Seed culture • - 100 ml LB in 500 ml shake flask • - overnight culture at 37 oC, 220rpm • Fermentation culture (7.5 L fermentor) • - inoculation volume: 10% (v/v) • - initial fermentation volume: 3 L • - temperature: 37 oC • - agitation speed: 600 rpm • - pH = 6.8-6.9, adjusted by 4 M (NH4OH:NaOH = 2:2)

  12. SUMMARY OF OXYGEN TRANSFER COEFFICIENT IN E. COLI FERMEMENTATION WITH AND WITHOUT MAGNETIC NANOPARTICLES

  13. SUMMARY AND CONCLUSIONS • Micro-Bioreactors Will Began to Have Impacts in Biotechnology Processes • Reducing Time in Process Development • Strain Selection • Medium Development • Product Quality in Mammalian Cell Culture • Nano-Technology Has Definite Future • Product Purification • Over-Coming Transport Barriers • Oxygen Transfer • Biocatalysis and Co-Factor Regeneration

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