E. coli O157:H7 Sampling Directive 10,010.1 Rev. 1

1. E. coli O157:H7 Sampling Directive 10,010.1 Rev. 1 In-Plant Retail Imports Talking points: Presentation can be found in Section 1 of the workbook, after the 2nd orange sheet of paper Presentation focuses mainly on new procedures for FSIS Inspection program personnel No current changes in the way we sample Focus is on ground beef and ground beef componentsTalking points: Presentation can be found in Section 1 of the workbook, after the 2nd orange sheet of paper Presentation focuses mainly on new procedures for FSIS Inspection program personnel No current changes in the way we sample Focus is on ground beef and ground beef components

2. Directive 10,010.1 Rev. 1 This directive provides new instructions for FSIS inspection program personnel on FSIS� E. coli O157:H7 policies Non-intact raw beef products contaminated with E. coli O157:H7 are adulterated Verifying control of presumptive and positive beef product Follow-up actions after an FSIS positive test result Talking points: Need to be aware if producing non-intact beef and trim, subject to sampling Identifying new control proceduresTalking points: Need to be aware if producing non-intact beef and trim, subject to sampling Identifying new control procedures

3. Plants Subject to Sampling Under this Directive All plants that produce Raw ground beef products Raw ground beef components Includes raw esophagus (weasand) meat, head meat, and cheek meat Beef manufacturing trimmings Beef from advanced meat recovery (AMR) systems Lean finely textured beef (LFTB) Talking points: Also relates to plants that supply to other plants for further processing (LFTB � low temperature rendering)Talking points: Also relates to plants that supply to other plants for further processing (LFTB � low temperature rendering)

4. Plants Subject to Sampling Under this Directive Raw beef patty components Includes components listed under Raw Ground Beef Components Partially defatted chopped beef (PDCB) Heart Partially defatted beef fatty tissue (PDBFT) Talking points: Beef patty mix can contain these and be marketed as beef patties Ground beef can�t have anything in it except spiceTalking points: Beef patty mix can contain these and be marketed as beef patties Ground beef can�t have anything in it except spice

5. Products Not Covered Products that contain another type of livestock or poultry species in addition to beef Beef sausage products Talking points: Consumers tend to eat these products more fully cooked as opposed to ground beef that consumers prefer to eat more rare Consumers tend to cook these products differently than they cook ground beef Talking points: Consumers tend to eat these products more fully cooked as opposed to ground beef that consumers prefer to eat more rare Consumers tend to cook these products differently than they cook ground beef

6. Sampling Procedures � In-Plant Office of Public Health Science (OPHS) Schedules sampling and Sends form 10,210-3 to inspection program personnel Talking points: This is a national sampling process Talking points: This is a national sampling process

7. Sampling Procedures � In-Plant Inspection program personnel Notify plant management of planned sampling and the reason for sampling (e.g., verification testing or follow-up sampling) Plant management can Elect to hold the sampled lot Talking points: Talk about small and very small plants, but emphasis is on public healthTalking points: Talk about small and very small plants, but emphasis is on public health

8. Sample Collection � In-Plant Inspection program personnel Collect samples from current day�s production Will not send sample until preshipment review is completed for that lot Talking points: Will not send until pre-shipment is completed, even if sample is pulled prior to thisTalking points: Will not send until pre-shipment is completed, even if sample is pulled prior to this

9. At Retail Office of Public Health Science (OPHS) schedules sampling The Office of Program Evaluation, Enforcement and Review (OPEER) receives FSIS Form 10,210-3, �Requested Sample Programs� Talking points:Talking points:

10. At Retail OPEER Program Investigator Makes every effort to notify the retail store the day before collection, so the retailer can hold the lot If this is not possible, arrives as close to the beginning of the grinding operation as possible Collects sample Records information on the source material Talking points:Talking points:

11. At Retail Program Investigators do not collect raw ground beef product that is received and sold as case-ready or Is only repackaged by the retail store Talking points: Discourage activities at retail that may impact the public healthTalking points: Discourage activities at retail that may impact the public health

12. At Retail Program Investigators do not collect product if the retail store only regrinds a product previously ground at an inspected plant Unless the retail store uses a process that might introduce E. coli O157:H7 into the product, such as adding store trim Talking points:Talking points:

13. Import Sampling Import inspector receives assignment from AIIS to collect sample Import inspection personnel: Notify the import establishment of the reason for sampling Importer, broker or applicant may elect to voluntarily hold the product until the results are reported Talking points:Talking points:

14. Additional Guidance I urge you to read carefully the new Directive 10,010.1 Rev. 1 Compliance Guidelines If you have questions, please call the Technical Services Center (TSC) at 1-800-233-3935 Talking points:Talking points:

15. Review � Flow Diagrams Three diagrams explain the flow of the sampling process for In-plant Retail Imports Talking points:Talking points: