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Bacterial Plant Pathogens. What are bacteria? Bacterial identification Generalized disease cycle Disease symptoms Disease control. Bacterial Plant Pathogens. Size (0.3-1.5 x 0.7-5 u m ). Generally single celled. Reproduce by simple fission. Prokaryotes. No membrane bound nucleus
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Bacterial Plant Pathogens • What are bacteria? • Bacterial identification • Generalized disease cycle • Disease symptoms • Disease control
Bacterial Plant Pathogens Size (0.3-1.5 x 0.7-5 um) Generally single celled Reproduce by simple fission
Prokaryotes No membrane bound nucleus or organelles Single circular chromosome Lack chlorophyll Most have cell wall Some are motile – flagellum (a)
Identification Structure cell shape colony appearance motility (flagella)
Identification Biochemical cell wall composition
Identification Respiration aerobic, anaerobic, facultative anaerobic Nutritional requirement (C, N, {Biolog}) Fatty acids {FAME} Serology Nucleic acids
Identification Pathogenicity
Plant Pathogenic Bacteria (for PLPA3004) • 8 genera of bacteria, 1 of uncertain ID • 2 genera of Mollicutes (lack cell walls • Approximately 50-60 spp. (200 pathovars {pv.}) • Pathovar – pathogenic on a specific host (crop)
Plant Pathogenic Bacteria (for PLPA3004) • Most are facultative parasites, • Except fastidious bacteria and mollicutes • Most are gram-, rod-shaped bacteria
Fastidious vascular bacteria • Vectored by leafhoppers, except Clavibacter • Unknown-phloem limited bacterium not culturable • Symptoms – stunting, leaf scalding, decline • Xylella – Pierce’s disease of grape
Mollicutes • Lack cell wall • Phloem limited • Transmitted by leafhoppers • Phytoplasmas – amorphous • Spiroplasmas - helical • Symptoms – yellows diseases, witches’ brooming, stunting • Sensitive to Tetracycline
Disease cycle Overseasoning 2 cycle o Reproduction Dissemination Disease development Penetration
Disease cycle Protected environment
Disease cycle • short distance • 1.Water • 2. Soil • 3. Tools and equipment • 4. Insects