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Femtosecond dynamics and near-field microscopy of chemical and biological objects

Institute of Chemical Physics Moscow, Russ i a. Femtosecond dynamics and near-field microscopy of chemical and biological objects. A.N. Petrukhin, O.M. Sarkisov. Femtosecond dynamics of bifunctional molecules. Near-field microscopy of lipofuscin granules from human eyes.

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Femtosecond dynamics and near-field microscopy of chemical and biological objects

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  1. Institute of Chemical Physics Moscow, Russia Femtosecond dynamics and near-field microscopy of chemical and biological objects A.N. Petrukhin, O.M. Sarkisov

  2. Femtosecond dynamics of bifunctional molecules. • Near-field microscopy of lipofuscin granules from human eyes.

  3. Photochromicreaction ESIPT reaction hn C-O bond cleavage: 250 - 400 fs Formation of intermediate X: 300-450 fs Formation of colored photoproduct (550-600 nm): 1 - 3 ps Excited state intramolecular proton transfer (ESIPT): < 80 fs Formation of twisted structure: 0.3 – 4.4 ps Formation of colored photoproduct (480-500 nm): 3.8 – 14 ps S.A. Antipin, A.N. Petrukhin, F.E. Gostev, V.S. Marevtsev,A.A. Titov, V.A. Barachevsky, Yu.P. Strokach, O.M.Sarkisov, Chem. Phys. Lett. 331 (2000) 378. F.E. Gostev,A.N. Petrukhin, A.A. Titov, V.A. Nadtochenko, A.V. Metelitsa, V.I. Minkin, O.M.Sarkisov, Rus. Chem. Bul.2004. 23 , 113-14.

  4. Bifunctional molecules: compounds 1-3 1: 2: 3: Does proton transfer take place?

  5. Experimental setup Pump: 405 nm, 40 fs Probe: Supercontinuum, 410-800 nm

  6. Photoinduced absorption dynamics

  7. Photoinduced absorption dynamics

  8. Product yield ratio dependence on pump energy Compounds 1,2 800 fs 3 ps ESIPT S1K* S1K S0K B S1** 120 fs 20 ps 400 fs S1* X B C-O cleavage S0K S0 S0K B B – photochromic product S0K – ESIPT product

  9. Product yield ratio dependence on pump energy Compound 3 21 ps 450 fs S2* X B B C-O cleavage 120 fs 850 fs 3 ps S1K* S1K S0K S1** S0K ESIPT S0 S0K B – photochromic product S0K– ESIPT product B

  10. Pump pulse chirp influence on wave packet motion Compound 1. Kinetic curve at 510 nm

  11. Pump pulse chirp influence on product yield ratio Time delay = 200 ps

  12. 2. Near-field microscopy of lipofuscin granules from human eyes.

  13. Near-field microscopy. Experimental Setup.

  14. 200 nm Topography Phase contrast Single LF granule. Atomic-force microscopy.

  15. Topography Transmission at 410 nm Single LF granule. Near-filed microscopy.

  16. Topography Fluorescence spectra Single LF granule. Near-filed microscopy.

  17. Two photon microscopy of LP granules

  18. Fluorescence decay of LH granules under 400 nm fs irradiation Fluorescence decay – epoxide formation?

  19. Prof. Oleg Sarkisov • Prof. Victor Nadtochenko • Andrey Titov • Fyodor Gostev • Andrey Petrukhin • Andrey Frolov • Alexander Sobennikov • Arseniy Aybushev • Artem Astafyev • Ivan Shelaev • Pavel Zolotavin Institute of Chemical Physics Moscow, Russia Our team: Thank you very much! Laser spectroscopy Lab femto.chph.ras.ru +7-095-939-7162

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