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NAJRAN UNIVERSITY College of Applied Medical Sciences

NAJRAN UNIVERSITY College of Applied Medical Sciences. General Microbiology Course Lecture No. 12. By. Dr. Ahmed Morad Asaad Associate Professor of Microbiology. Sterilization and disinfection Sterilization: Complete inactivation of all forms of microorganisms

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NAJRAN UNIVERSITY College of Applied Medical Sciences

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  1. NAJRAN UNIVERSITY College of Applied Medical Sciences General Microbiology Course Lecture No. 12 By Dr. Ahmed MoradAsaad Associate Professor of Microbiology

  2. Sterilization and disinfection • Sterilization: Complete inactivation of all forms of microorganisms • Disinfection: Inactivation of all forms of microorganisms except bacterial spores. • Antiseptic: Compound applied to human tissue to kill or inhibit microorganisms

  3. Disinfectant: Compounds that kill microorganisms but they are harmful to human tissues (inanimate surfaces) • High level disinfectant: Kill bacteria, viruses, mycobacteria and most spores • Low level disinfectant: Kill bacteria and most viruses (not mycobacteria or spores)

  4. Physical means of sterilization • Heat Radiation Filtration • Dry heat Moist heat • - Red heat • -Hot air oven • -Incineration< 100ºC at 100ºC > 100ºC • Pasteurization Boiling Autoclave • Tyndallization

  5. TUTORIALS • Sterilization by radiation • Sterilization by filtration • Disinfectants

  6. Antimicrobial agents • TUTORIALS • Development of resistance (TUTORIAL) • Non-genetic OR genetic drug resistance

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