1 / 16

Invasive and endemic Vibrio parahaemolyticus lineages in the Northeast US

Invasive and endemic Vibrio parahaemolyticus lineages in the Northeast US. Feng Xu , Ashley Marcinkiewicz , Narjol Gonzalez-Escalona, Vaughn Cooper, Steve Jones, & Cheryl A. Whistler. Starting at the end (Our conclusions).

wardgloria
Download Presentation

Invasive and endemic Vibrio parahaemolyticus lineages in the Northeast US

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Invasive and endemic Vibrio parahaemolyticuslineages in the Northeast US Feng Xu, Ashley Marcinkiewicz, Narjol Gonzalez-Escalona, Vaughn Cooper, Steve Jones, & Cheryl A. Whistler

  2. Starting at the end(Our conclusions) • Most infections in the Northeast are caused by two resident lineages: Pacific-originating ST36 (O4:K12) and Atlantic endemic ST631 (O11:KUT). • The Atlantic US Coast has at least 3 distinct and recognizable (by their genetic fingerprint) ST36 populations, each founded by a single bacterium. • ST631 is the first reported major endemic pathogen to have evolved from the North Atlantic Vp population, and it acquired its “pathogenicity island” from a Pacific strain (potentially from ST36). • Other lineages derived from the Pacific, the Gulf of Mexico, Asia, and even the Baltic Sea have been implicated in local infections.

  3. Disease is dominated by relatively few unrelated strains Proportion of Infection by Sequence Type Xu et al., 2015 and unpublished

  4. Disease is dominated by relatively few unrelated strains Proportion of Infection by Sequence Type Asia: ST3, ST8, ST120 Xu et al., 2015 and unpublished

  5. Disease is dominated by relatively few unrelated strains Proportion of Infection by Sequence Type Asia: ST3, ST8, ST120 PNW: ST43, ST636 GOM: ST110, ST34, ST308 Xu et al., 2015 and unpublished

  6. Regional ST36 sub-populations are distinctive

  7. Rapid identification of isolates by subpopulation PI.36 PI.family PI.26 NEA tlh control

  8. Clade II ST631 is a mixed population

  9. Virulence genes are contained in one of three types of pathogenicity islands

  10. Most emergent pathogens harbor a similar hybrid-type VPaIγ

  11. Pacific invasive and Atlantic native ST631 have the same tdh3 allele (Gulf of Mexico derived lineages typically have tdh5)

  12. VPaIγ of ST631 clade II shares a common origin with VPaIγ of invasive Pacific lineages

  13. Strain- and island-specific MPN-qPCR ST36 ST631 Cullen, J.J. and H.L. MacIntyre (2015). J. Appl. Phycol. DOI 10.1007/s10811-015-0601-x. http://creativecommons.org/licenses/by/4.0/

  14. Re-Conclusions • Relatively few strains cause the majority of infections in the Northeast US • Several originated in the Pacific Ocean • Most Northeast US emergent pathogenic lineages share a similar pathogenicity island • The most prevalent Atlantic endemic lineage acquired virulence through lateral exchange with non-Atlantic natives • Surveillance of pathogens could be improved by targeted enumeration of prevalent strains or their associated island

  15. Acknowledgements • MA Department of Public Health • MA Division of Marine Fisheries • C. Schillaci, Tracy Stiles, M. Hickey (ohters) • NH Department of Public Health • ME CDC & Department of Marine Resources • CT Department of Agriculture • Bureau of Aquaculture • K. DeRosia-Banick, Joe DeCrescenzo • FDA • J. Jones (PCR Queen) cnfengxu@gmail.com

  16. Origin and evolution of major resident northwest Atlantic pathogenic lineages

More Related