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H2B

0.5. 0.4. 0.3. HAT activity. 0.2. 2N. 4N. 0.1. R120. R90. R75. R60. R45. DNA Contents. R30. R0. Asyn. 0.0. Asyn. R0. R30. R45. R60. R75. R90. R120. Time after release from nocodazole treatment (min). Time after release from nocodazole treatment (min).

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H2B

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  1. 0.5 0.4 0.3 HAT activity 0.2 2N 4N 0.1 R120 R90 R75 R60 R45 DNA Contents R30 R0 Asyn 0.0 Asyn R0 R30 R45 R60 R75 R90 R120 Time after release from nocodazole treatment (min) Time after release from nocodazole treatment (min) Time after release from Thy-DB (h) Asyn 10.5 2.5 8.5 9.5 10 12 13 11 0 5 6 7 8 9 H3 H2B H2A H4 P-H3 H3AcK9 H3AcK14 Actin Mitosis G1 G1/S (Thy DB) G2 (Eto) G2/M Pro Pro Meta Meta Early Ana Ana Ana Late Ana NEBD DNA aAcK Pericentrin Merge DNA Tubulin aAcK Merge DNA Tubulin aAcK Merge Prometaphase Metaphase Anaphase p300 shRNA Con shRNA Supplemental Figure S4 a b 13 12 11 10.5 10 9.5 9 8.5 8 2N 7 6 5 4N Time after release from Thy-DB (h) 2.5 0 DNA Contents Asyn c d

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