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Rotifers

Rotifers. their role and production requirements. Reproduction. Mass production. Size. Mobility. Tolerance. Bio - encapsulation. Life cycle B. plicatilis. Rotifer production – optimal culture and enrichment conditions. Tank: Any Size/Shape (2-6 m 3 )

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Rotifers

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  1. Rotifers their role and production requirements Live food aquaculture training course www.aquatrain.org

  2. Reproduction Mass production Size Mobility Tolerance Bio-encapsulation Live food aquaculture training course www.aquatrain.org

  3. Life cycle B. plicatilis Live food aquaculture training course www.aquatrain.org

  4. Rotifer production – optimal culture and enrichment conditions • Tank: Any Size/Shape (2-6 m3) • Temp.: 25-27 °C (Preheated Water/Heat Exchange) • Salinity: 18-25 ppt • Oxygen: 5-7 ppm (Pure Oxygen) • pH: 7.5-8.5 • NH3/NH4+: < 10 mg/l • NH3: < 1 mg/l • Water: Filtered (1 m), UV, Chlorinated/Neutralized • Aeration - Reasonably Strong - Airstones • Illumination: Low Light Conditions • Floc traps • Batch Culture: 3-5 days Live food aquaculture training course www.aquatrain.org

  5. Rotifer production in 3m3 tanks Live food aquaculture training course www.aquatrain.org

  6. Sea bream General assumptions 1) Av. daily rotifer consumption/m3 larval volume (M) 10 2) Rotifer production volume/100M daily production (m3) 1,5 3) Period of rotifer feeding (days) 20 4) Culture Selco needed per 100M rotifers produced (kg) 0,2 5) Culture Selco price (drs/kg) 11234 Annual production target (2g juveniles) Requirements 2.000.000 4.000.000 6.000.000 10.000.000 No of larval batches 5 5 5 5 Initial larval rearing vol (m3) 62 123 185 308 Daily rotifer requirement (M) 615 1.231 1.846 3.077 Rotifer production volume (m3) 9 18 28 46 No of 2.8m3 tanks needed 3 7 10 16 No of 4m3 tanks required 0 0 0 0 Total rotifer production volume (m3) 8 20 28 45 Total Culture Selco requirement (kg) 123 246 369 615 Cost of Culture Selco (Mdrs/yr) 1,38 2,77 4,15 6,91 Low density rotifer culture Live food aquaculture training course www.aquatrain.org

  7. Rotifer culture using algae • excellent results with algae but expensive • time consuming (culture of algae + counting) • availability of algae • quality of the rotifers dependent on quality of the algae Live food aquaculture training course www.aquatrain.org

  8. variable results in rotifer survival depending on the quality of algae and freshness of the yeast unexpected mortalities (total crashes) due to lack of standardisation variability in rotifer quality (nutrition, microbiology) Rotifer culture using algae and yeast Live food aquaculture training course www.aquatrain.org

  9. Batch culture of rotifers on algae and yeast Re-inoculation Culture period 6-10 days f e e d i n g harvest drain culture water Live food aquaculture training course www.aquatrain.org

  10. Sea bream General assumptions 1) Av. daily rotifer consumption/m3 larval volume (M) 10 2) Rotifer production volume/100M daily production (m3) 0,4 3) Period of rotifer feeding (days) 20 4) Culture Selco 3000 needed per 100M rotifers produced (kg) 0,20 5) Culture Selco price (drs/kg) Annual production target (2g juveniles) Requirements 2.000.000 4.000.000 6.000.000 10.000.000 No of larval batches 5 5 5 5 Initial larval rearing vol (m3) 62 123 185 308 Daily rotifer requirement (M) 615 1.231 1.846 3.077 Rotifer production volume (m3) 2 5 7 12 No of 2.8m3 tanks needed 3 7 10 16 No of 4m3 tanks required 0 0 0 0 Total rotifer production volume (m3) 8 20 28 45 Total Culture Selco 3000 requirement (kg) 123 246 369 615 Cost of Culture Selco (Mdrs/yr) 0,00 0,00 0,00 0,00 Medium density rotifer culture Live food aquaculture training course www.aquatrain.org

  11. Rotifer growth comparison CS v’s CS3000 Live food aquaculture training course www.aquatrain.org

  12. Specific Growth Rate in MD batch culture Live food aquaculture training course www.aquatrain.org

  13. Method of determining rotifer growth and condition– mean specific growth rates SGR = ln (Final Number)-ln (Initial number) No. of days of culture ln = natural log Useful for:- Following feeding experiments Quantitatively assessing culture stability Live food aquaculture training course www.aquatrain.org

  14. Rotifer production methodologiesCulture Selco 3000 and O2 Live food aquaculture training course www.aquatrain.org

  15. M: Masters A: Culture (2-6 m3) B: Inoculum (500 R/ml) C: Enrichment (500-1000 R/ml) Larval Tanks Rotifer Production M M2 A 70% 30% B C Live food aquaculture training course www.aquatrain.org

  16. Culture Selco 3000 strategy Live food aquaculture training course www.aquatrain.org

  17. Traditional rotifer batch culture Live food aquaculture training course www.aquatrain.org

  18. High density rotifer production system Live food aquaculture training course www.aquatrain.org

  19. Rotifer harvesting Live food aquaculture training course www.aquatrain.org

  20. Harvesting of rotifers Live food aquaculture training course www.aquatrain.org

  21. Transfer of rotifers Live food aquaculture training course www.aquatrain.org

  22. Recirculated ultra-high density rotifer culture ~5000 rotifers + /ml Live food aquaculture training course www.aquatrain.org

  23. Commercially available rotifer and artemia cultivation and enrichment products Live food aquaculture training course www.aquatrain.org

  24. Fatty acid enrichment levels in Rotifers Live food aquaculture training course www.aquatrain.org

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