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Biotech

Biotech. Southern Blotting. Through a series of steps, DNA that has been separated by electrophoresis is applied to a membrane of nylon or nitrocellulose . PCR.

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Biotech

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  1. Biotech

  2. Southern Blotting • Through a series of steps, DNA that has been separated by electrophoresis is applied to a membrane of nylon or nitrocellulose.

  3. PCR • This is the polymerase chain reaction. It is a technique to multiply a sample of DNA many times in a short period of time. It supplies the scientist with sufficient DNA for further testing. • http://wps.prenhall.com/wps/media/objects/487/498929/CDA12_2/CDA12_2a/CDA12_2a.htm

  4. PCR diagram

  5. DNA fingerprinting (RFLP analysis) • RFLP is restriction fragment length polymorphism. This is the idea that each person’s DNA would cut up into different sized fragments. Thus the pattern produced by electrophoresis of those patterns would be unique to the person. • Often probes are used to detect specific fragments, so the pattern is not so “crowded” to see.

  6. DNA fingerprinting analysis • http://www.biology.arizona.edu/human_Bio/problem_sets/DNA_forensics_1/02Q.html • Do problems 2-4 to practice the interpretation of DNA fingerprints.

  7. Key terms to define • Genomic Library • DNA sequencing • cDNA • Human genome project • Primer • Hybridization • Stem cells

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