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MLAB 1415: Hematology

MLAB 1415: Hematology. Chapter 7: Leukocytes Part Two. MONOCYTES. Monopoiesis - the production and development of monocytes, macrophages and their precursors. Monocytes are produced in the bone marrow. MONOCYTES. Differentiation & maturation Produced from CFU-GM

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MLAB 1415: Hematology

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  1. MLAB 1415: Hematology Chapter 7: Leukocytes Part Two

  2. MONOCYTES • Monopoiesis - the production and development of monocytes, macrophages and their precursors. Monocytes are produced in the bone marrow.

  3. MONOCYTES • Differentiation & maturation • Produced from CFU-GM • Can further differentiate into macrophages in the tissues • Lifespan • P.B ~ 8 hours • Tissue • Regulation • Production primarily regulated by cytokines • GM-CSF • IL-3 • M-CSF

  4. Monocytes/Macrophages • Function • Protect the host from infectious agents or pathogens via innate and adaptive immune response • Phagocytosis • Scavengers

  5. Maturation of the monocytic series • Monoblast • Size: 12-20µm • Cytoplasm • Blue, moderate amount • No granules • Nucleus • Round, oval or slightly folded • Eccentric • Reddish-purple • Chromatin fine and lacy • Vacuoles may be present • 1-2 nucleoli • N:C ratio = 4:1

  6. Maturation of the monocytic series • Promonocyte (difficult to discern) • Size: 12-20µm • Cytoplasm • Paler gray-blue and abundant • “Bleb-like” pseudopods at periphery • May contain fine red “dust-like” particles, azurophilic granules • Vacuoles may be present • Nucleus • Round with chromatin creases or brain-like folding • Central • Reddish-purple with light clumping of chromatin • 0-2 nucleoli • N:C ratio = 3:1 or 2:1

  7. Promonocyte

  8. Maturation of the monocytic series • Mature monocyte • Normal in circulation: 2-9% • Size: 12-20µm • Cytoplasm • Pale gray-blue and abundant • Bleb-like pseudopods at periphery, variable shape • Numerous fine pale red dustlike granule scattered throughout • Vacuoles common • Nucleus • Increased folding or elongated • Central • Purplish with finer clumped chromatin • No nucleoli • N:C ratio = Variable

  9. Monocyte

  10. Monocytes…THINK…. Fine, Lacy Chromatin Ground Glass Cytoplasm

  11. Macrophages • Once the monocyte enters the tissues, it becomes a macrophage • Size:15-80µm • Cytoplasm • Gray-blue and abundant • Ragged edges • Vacuoles common • Nucleus • Round with netlike appearance • Purplish with finer clumped chromatin • Nucleoli appear

  12. Macrophages in Various Tissues • Macrophage alias’ • Histiocytes in the loose connective tissue • Kupffer cells in the liver • Osteoclasts in the bone • Microglial cells in the nervous system • Also scattered throughout the body in all organ tissues (spleen, lung, abdomen, etc)

  13. Macrophage

  14. References • http://winsorb.com/silica-gel.php • http://image.bloodline.net/stories/storyReader$1075 • MacKenzie, S., & Lynne, W. J. (2010). Clinical Laboratory Hematology. Upper Saddle River: Pearson Education, Inc.

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