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NEPHELOMETRY AND TURBIDIMETRY

NEPHELOMETRY AND TURBIDIMETRY. Sweta hadiya Shivani patel Krishna chauhan Sultan lakhani Divyesh patel. INTRODUCTION. When light strikes a particle in solution, light will be scattered or reflected.

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NEPHELOMETRY AND TURBIDIMETRY

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  1. NEPHELOMETRY AND TURBIDIMETRY Sweta hadiya Shivani patel Krishna chauhan Sultan lakhani Divyesh patel

  2. INTRODUCTION • When light strikes a particle in solution, light will be scattered or reflected. • The amount of light scattered is proportional to the concentration of insoluble particle.

  3. THEORY • Scattered light may be measured by • Turbidimetry • Nephelometry

  4. Colorimeter principle

  5. NEPHELOMETRY • Detect low concentration of particles • Highly sensitive • High Accuracy • Intensity of scatted light measured at 90 degree, 35 degree & 135 degree • Separate Instrument require • TURBIDIMETRY • Detect only high concentration of particles • Less sensitive • Low Accuracy • Intensity of scatted light measured at 1800 • Can be done on any biochemistry analyzer with colorimeter principle

  6. Turbidometric measurements are made at 180o from the incident light beam. • In Nephelometry, the intensity of the scattered light is measured at right angles (90 degree) to the incident light beam, ( light which pass through the solution). • It can measure at other angle also • 35 degree • 35 to 135 degree

  7. Light scattering dependent on : • Particle size • Molecular weight of particles • Concentration of particles • Wavelength of the light • Path length of cuvette

  8. Tyndall Effect • Scattering of light occur due to particles. • High wavelength light = Less Scattering • Low Wavelength light = More Scattering. • For Turbidometery & Nephometery • = Best Filter 420 nm (Blue colour)

  9. Light Scattering Phenomenon – Tyndall effect

  10. Light Scattering Phenomenon Tyndall effect • Sky Looks Blue • Sun through all wave length light • But Blue light (420 nm ) make maximum scattering in “ AASHMAN” • So Only colour visiable in sky is “Blue” • Sun Looks Red • Sun through all wave length light • But Red light (630 nm ) make minimum scattering • So Red colour directly reach to “PRUTHVI”

  11. Light Scattering Phenomenon – Tyndall effect

  12. APPLICATIONS • Quantitative & qualitative estimation of following is does through turbidometery and Nephalometrty • 1. CRP (C-reactive protein) • 2. RF (Rheumatoid factor) • 3. ASO (Anti-streptolysin-o) • Albumine • Haptoglobin • Blood group

  13. Thanks For Listening Silently You Are Free To Through Any Thing

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