NOTICE!!! PRICES ARE SUBJECT TO CHANGE.

1. NOTICE!!! PRICES ARE SUBJECT TO CHANGE. CEO Networking Luncheon

2. CHEMISTRY SERVICES at UMBC by WILLIAM R. LaCOURSE Department of Chemistry and Biochemistry University of Maryland, Baltimore County 1000 Hilltop Circle Baltimore, MD 21250 PH: 410-455-2105 FX: 410-455-2608 Email: lacourse@umbc.edu CEO Networking Luncheon

3. GUIDE TO SHOPS AND SERVICES • Mass Spectrometry Facility • Nuclear Magnetic Resonance Facility • Other Instrumentation • Shops – Glass, Electronic & Machine • Contract Research • Consultancy CEO Networking Luncheon

4. OVERVIEW • All instrumentation and shops • owned and operated by the Dept. of Chemistry & Biochemistry • UMBC researchers/outside customers - charged fees for use • Fees – pay costs of operation (NOT FOR PROFIT) • Costs • General • housing, utilities, insurance, service contracts, maintenance and repairs • Specific examples • MS: gases, pumps, software, and supplies • NMR: cryogens, software, and supplies • Technicians - ‘soft’ money positions • Maintain and operate instrumentation and shops • Salary support • Cost of service – included in fees • Contract research • Contract teaching • Limited departmental subsidy CEO Networking Luncheon

5. MASS SPECTROMETRY (MS) FACILITY • PURPOSE • a resource offering service, training and instrumentation with the primary aim of providing investigators with access to contemporary mass spectrometry instrumentation and expertise. • Biomolecule Applications • Protein Chemistry • Protein ID by peptide mass fingerprinting using MALDI • Determination of protein MW • Sample composition validation • Protein purification assessment • Oligonucleotide Analysis • Identity and purity validation • Small Molecules Applications • Structure elucidation by tandem mass spectrometry (MSn) • Elemental composition determination through accurate mass measurement • General Analytical Services • LC-MS and GC-MS purity assessment • Impurities and degradants profiling CEO Networking Luncheon

6. MS INSTRUMENTATION • Bruker AUTOFLEX (2002) MALDI Time of flight • Range: Linear: > 250 kDa Reflectron: > 5,000 Da • Resolution Linear: > 4,000 (FWHM) Reflectron: >10,000 (FWHM) • Characterization of proteins, oligonucleotides and other polymers. • Characterization and identification of proteins by peptide mass mapping and sequencing. • Bruker ESQUIRE 3000 (2002) ESI, APCI, Ion Trap • Range: 50-3000 • Resolution: Nominal mass measurement capability throughout the entire m/z range • Electrospray ionization (ESI), atmospheric press. chem. ionization (APCI) • Analysis of non-volatile and thermally-labile compounds; protein mass determination and digest analysis; peptide sequencing. • Shimadzu GC-17A/QP-5000 (1993+) EI, Quadrapole • Mass Range: 10-700 • Analysis of volatile (GC amendable) compounds • JEOL HX110/HX100 (1986) EI, CI, FD, FAB, ESI Sector • Range: 1- >10,000 Resolution: Up to 100,000 • ESI Applications • Mostly used for exact mass measurement using FAB (Fast Atom Bombardment) CEO Networking Luncheon

7. SUBMITTED SAMPLE ANALYSIS • UMBC researchers and outside users can submit samples for the following services: • FAB unit resolution MS • FAB accurate mass measurement and empirical formula determination. • MALDI-TOF • ESI or APCI direct infusion • ESI or APCI LC-MS with HPLC (reverse and normal phase) separation • GC-MS CEO Networking Luncheon

8. SAMPLE SUBMISSION • Identify the most suitable approach which will provide information you are looking for • Become familiar with sample preparation guidelines and make sure that the sample(s) meet the guidelines • Fill out a sample submission form • Deliver samples accompanied by the sample submission form(s). • It is suggested that we receive samples as solid material (just enough to see it) or neat liquid (several ul) for FAB, ESI, APCI. For MALDI-TOF analysis submit ~10 pmol of peptide, protein or oligonucleotide (>100 pmol) dry or in minimal (several µl) volume of solvent. CEO Networking Luncheon

9. MS FACILITY RATES • MALDITOF: $55/sample or $130/h • ESI or APCI direct infusion $60/sample or 130/h • ESI or APCI LC-MS separation $100/h • GC-MS $80/h • FAB Lo-Res: $55/sample Hi-Res: $90/sample • Time is rounded to the next hour/no method development • Method Development – costs handled on an individual basis. CEO Networking Luncheon

10. WORKSHOPS • GC Mass Spectrometry (GC-MS) Workshop: 1 day • Topics will include: • Instrument overview: Gas chromatograph, Quadrapole MS, ion detection, vacuum system • EI source and spectra interpretation • Analysis by EI full scan and selected ion monitoring mode • Quantitation of unknown samples • Hands-on sample running and covering of applications requests • Fees -$295.00 per participant • includes course materials, lab supplies and instrument time. • Participants will have ample opportunity to discuss their applications. • Also the attendees will receive guidance on using the facility instruments and expertise to meet their goals. CEO Networking Luncheon

11. WORKSHOPS • MALDI Time of Fight Mass Spectrometry (MALDI-TOF) Workshop (2 days) • Participants will: • Learn basic theory necessary for intelligent instrument operation. • Receive training on instrument operation and data analysis. • Prepare and analyze several representative samples. • Learn about MALDI-TOF capabilities and limitations due to instrument, sample preparation and substrate issues. • Workshop schedule: • Day 1 • Facility tour and instrument operation demonstration. • Mass spectrometry primer, MALDI overview and basic theory of TOF instrumentation. • Instrument operation overview. Participants prepare samples and acquire data with assistance as necessary. Data analysis software overview followed by analysis of acquired spectra. • Day 2 • First day material review. • A detailed study on the effect of sample concentration and level of impurities on data quality. • Fees -$495.00 per participant • includes course materials, lab supplies and instrument time. • Participants will have ample opportunity to discuss their applications. • attendees will receive guidance on using the facility instruments and expertise to meet their goals. CEO Networking Luncheon

12. WORKSHOPS • Liquid Chromatography-Mass Spectrometry (LC-MS) Workshop (2 days) • Participants will: • Learn basic theory governing instrument operation. • Receive training on instrument operation and data analysis. • Prepare and analyze several representative samples. • Learn about LC-MS capabilities and limitations due to instrument, sample preparation and matrix issues = WISDOM. • Workshop Schedule: • Day 1 • Facility tour and instrument operation demonstration. • Mass spectrometry primer, electrospray ionization overview and basic theory of ion traps. • Instrument operation overview. Participants prepare samples and acquire data with assistance as necessary. Data analysis software overview followed by analysis of acquired spectra. • Day 2 • First day material review. • A discussion on HPLC requirements: Column and mobile phase options and an Introduction to MS/MS. • Setting up a MS/MS experiment followed by data analysis and discussion. • Fees - $495.00 per participant • includes course materials, lab supplies and instrument time. • Participants will have ample opportunity to discuss their applications. • attendees will receive guidance on using the facility instruments and expertise to meet their goals. CEO Networking Luncheon

13. MS FACILITY MANAGER Dr. Alexei Gapeev Office: Room 140-A, Meyerhoff Building Office Phone : 410-455-6251 Fax: 410-455-2608 E-mail address: gapeev@umbc.edu Initial Contact should be made through Dr. Bill LaCourse. CEO Networking Luncheon

14. NMR FACILITY • Seven (7) NMR spectrometers • ranging from 800MHz to 200MHz  • Howard Hughes Medical Institute – biomolecular studies • Three (3) Bruker NMR spectrometers • one DMX800 equipped with cryoprobe • two DMX600, one equipped with cryoprobe. • Graduate and Undergraduate Research – small molecule focus • Bruker DRX500 with cryoprobe • JEOL ECX400 • GE QE300 • Undergraduate Education - teaching • Varian Mercury 200 CEO Networking Luncheon

15. NMR FACILITY • PURPOSE • serve as a resource offering service, training and instrumentation with the primary aim of providing investigators with access to contemporary NMR instrumentation and expertise. • The NMR Facility - commercial analytical services • Focus: 1H, 13C, 15N • Rates • 500MHz: • 50$/hour(Commercial) • 100$/hour(Consultation) • 150$/hour(Commercial, Assisted) • 400MHz: • 50$/hour(Commercial) • 100$/hour(Consultation) • 150$/hour(Commercial, Assisted) CEO Networking Luncheon

16. NMR FACILITY MANAGER Dr. David Yeh Office: Room 247B, Meyerhoff Building Office Phone : 410-455-1031 Fax: 410-455-2608 E-mail address: deok@umbc.edu Initial Contact should be made through Dr. Bill LaCourse. CEO Networking Luncheon

17. OTHER INSTRUMENTATION • Specialty Instrumentation & Techniques • Examples • X-RAY Diffractometer • EPR Spectrometer • Absorption and Luminescence spectrophotometers • ThermoNicolet FT-IRspectrometer • Circular Dichroism • Calorimetry • Electrochemical Analysis • Operation – University personnel only • Access – may be limited • Fees – individually contracted • Contact – Dr. Bill LaCourse CEO Networking Luncheon

18. GLASS SHOP • PURPOSE • Custom glassblowing services for the UMBC community and off-campus clients. • DESCRIPTION • Expert scientific glassware and glass component repair, design assistance and manufacture of novel glass pieces, and general glassblowing services. • RATES • $35.00/hour plus the cost of materials. • CONTACT • Mr. Anthony Baney, Glass Artisan • Initial Contact – Dr. Bill LaCourse CEO Networking Luncheon

19. ELECTRONIC & MACHINE SHOPS • PURPOSE • Electronic repair and custom machining services for the UMBC community and off-campus clients • RATES • $35.00/hour plus the cost of materials. • CONTACTS • Electronic services • Mr. Nikollai Galitsky, Electronic Technician • Initial Contact – Dr. Bill LaCourse • Machine shop services • Mr. John Cataldi, Machinist • Initial Contact – Dr. Bill LaCourse CEO Networking Luncheon

20. CONSULTANSHIP/CONTRACT RESEARCH • Faculty - a group of faculty members who are leaders in scientific research. • Their activity is reflected in the amount of outside funding for research in the department, currently over $4 million per year. • Center for Structural Biology • Howard Hughes Medical Institute • Newly Renovated Building • Modern conference and seminar rooms. • Extensive array of tools for modern chemical and biochemical research • Calorimetry, chromatography, stopped-flow and temperature-jump kinetics, transient laser spectroscopy, NMRs, X-band CW electron paramagnetic resonance spectrometry, circular dichroism, X-ray diffraction, infrared spectroscopy, atomic absorption- and gas chromatography-mass spectrometry, and Fourier transform ion cyclotron resonance mass spectrometry apparatus, as well as extensive molecular modeling and computational chemistry facilities. • Ready access to literature • Department reference room • Albin O. Kuhn library - 2,500 volumes of text/> 150 chemistry and biochemistry periodicals on-site • Modern e-journal browsing CEO Networking Luncheon

21. CONTACT INFORMATION • Visit our website • www.umbc.edu/chem-biochem/Shops.html • Head of Business Operations • Dr. Bill LaCourse • Utilize departmental resources to support research, economic viability, and outreach. CEO Networking Luncheon