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PhD 2016

PATHOLOGICAL STUDIES ON INDUCED HEPATIC CIRRHOSIS WITH EVALUATION OF SOME HEPATOPROTECTIVE MATERIALS IN RATS. PhD 2016. بسم الله الرحمن الرحيم. "قَالُواْ سُبْحَانَكَ لاَ عِلْمَ لَنَا إِلاَّ مَا عَلَّمْتَنَا إِنَّكَ أَنتَ الْعَلِيمُ الْحَكِيمُ " (32)- البقرة.

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PhD 2016

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  1. PATHOLOGICAL STUDIES ON INDUCED HEPATIC CIRRHOSIS WITH EVALUATION OF SOME HEPATOPROTECTIVE MATERIALS IN RATS PhD 2016

  2. بسم الله الرحمن الرحيم "قَالُواْ سُبْحَانَكَ لاَ عِلْمَ لَنَا إِلاَّ مَا عَلَّمْتَنَا إِنَّكَ أَنتَ الْعَلِيمُ الْحَكِيمُ " • (32)- البقرة

  3. PATHOLOGICAL STUDIES ON INDUCED HEPATIC CIRRHOSIS WITH EVALUATION OF SOME HEPATOPROTECTIVE MATERIALS IN RATS Asmaa Khairy Mohammed Al-Mokaddem MSc. (2013) Supervision

  4. Introduction

  5. Introduction • The liver is one of the most important organs of the body. It performs a fundamental role in the regulation of diverse physiological processes, and its activity is related to different vital functions, such as metabolism, secretion, and storage. • Cirrhosisis a diffuse disorganization of normal hepatic structure by extensive fibrosis associated with regenerative nodules. Advanced cirrhosis leads to major alterations in the hepatic vascular bed and is usually irreversible • Fibrosisconsists of the accumulation of extracellular matrix (ECM) proteins, mainly fibrillar collagen, as the result of both increased ECM synthesis and decreased degradation of matrix.

  6. Vascular and architectural alterations in Cirrhosis

  7. Interventions in Cirrhosis management • There is no standard treatment for liver fibrosis, there are several therapeutic approaches to control liver fibrosis. • The ideal antifibrotic therapy would be one that is liver-specific, well tolerated when administered for prolonged periods of time, and effective in attenuating excessive collagen deposition without affecting normal ECM synthesis • Antioxidantsare the most promising drugs in treatment or prevention of liver fibrosis. They inhibit HSC activation, protect hepatocytes.

  8. Natural products with Hepatoprotective effect 1 Silymarin (Silybummarianum)

  9. Natural products with Hepatoprotective effect 2 Propolis (Bee Glue)

  10. Natural products with Hepatoprotective effect 3 Spirulina (Spirulina platensis)

  11. Natural products with Hepatoprotective effect 4 Naringenin

  12. Aim of Work Histopathological examination and scoring of TAA-Induced hepatic cirrhosis as a reliable model of experimentally induced cirrhosis and correlation between histopathological scores and serum biomarkers. Evaluation of the hepatoprotective effects of some natural agents (Silymarin, Propolis, Spirulina and Naringenin) against TAA-induced hepatic cirrhosis.

  13. Materials & Methods

  14. Materials & Methods (Chemicals) 1 2 3 5 4 Thioacetamide (TAA) Silymarin Propolis Naringenin Spirulina 100 mg/kg.Bwt – Daily 50 mg/kg.Bwt – Daily 300 mg/kg.Bwt – Daily 100 mg/kg.Bwt – Daily 200 mg/kg.Bwt – Twice/ week + Distilled Water

  15. Materials & Methods (Animals) 300 Male 10 Groups 120-150 Grams Sprague Dawley

  16. 3 Materials & Methods (Samples) Samples Sacrificed weekly

  17. Materials & Methods Samples (Samples) • Liver Function tests (AST, AlP, GGT) • Kidney Function tests (BUN, Creatinine) Blood Serum

  18. Materials & Methods Samples (Samples) 10% NBF Liver -20o C

  19. Materials & Methods Samples (Samples)

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