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Control of DNA replication. Replicon Origins and terminators Solutions to the “end problem” (telomeres) Cellular control mechanisms. 3 stages to replication. Initiation : begin at a specific site, e.g. oriC for E. coli . Elongation : movement of the replication fork
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Control of DNA replication Replicon Origins and terminators Solutions to the “end problem” (telomeres) Cellular control mechanisms
3 stages to replication • Initiation: begin at a specific site, e.g. oriC for E. coli. • Elongation: movement of the replication fork • Termination: at ter sites for E. coli
Replicon = unit that controls replication Replicator: cis-acting DNA sequence required for initiation; defined genetically Origin: site at which DNA replication initiates; defined biochemically Initiator: protein needed for initiation, acts in trans
Theta-form replication intermediates visualized in EM for polyoma virus B. Hirt
For completed molecules, label appears first in the fragments of DNA synthesized last
Labeling of completed DNA molecules can map replication origins Dana and Natahans, 1972, PNAS: map the replication origin of SV40 by labeling replicating molecules for increasing periods of time, isolating complete molecules, digesting with Hind restriction endonucleases, and determining which fragments have the most radioactivity.
Data from labeling completed DNAs Relative amount of pulse label Fragment5 min10 min15 min A 1.0 1.0 1.0 B 3.9 3.0 2.3 C 0 0.75 0.75 D 0.92 0.86 1.1 E 1.8 2.0 1.7 F 4.0 3.1 2.4 G 5.4 4.2 2.6 H 1.7 2.5 2.0 I 2.7 3.0 2.2 J 4.9 3.7 2.6 K 2.4 2.9 1.9
Replicating molecules have different shapes generated by replication bubbles and forks
Bubble arcs on 2-D gels 1st dimension: size 2nd dimension: size and SHAPE Twice unit length Unit length A fragment containing an origin will have one or two replication forks moving through it, generating bubbles of increasing size. These will be detected as bubble arcs on 2-D gels of the replicating DNA, when that region is used as a hybridization probe.
Y-arcs on 2-D gels of replicating molecules 1st dimension: separate by size 2nd dimension: separate by size and SHAPE A replication fork moving through a region will show a Y-arc on 2-D gels of the replicating DNA, when that region is used as a hybridization probe. Brewer and Fangman, 1987
Restriction fragments: origin termination Fork movement Example of analysis of a replicon using 2-D gels
Positions of oriC and ter in E. coli Replication fork 2
Features of oriC • oriC was identified by its ability to confer autonomous replication on a DNA molecule, thus it is a replicator. • Studies show that chromosomal DNA synthesis initiates at oriC, thus it is also an origin of replication. • Replication from oriC is bidirectional.
245 bp long 4 copies of a 9 bp repeat 3 copies of a 13 bp repeat 11 GATC motifs Structure of oriC 13 13 13 9 9 9 9 1 GGATCCGGAT AAAACATGGT GATTGCCTCG CATAACGCGG TATGAAAATG GATTGAAGCC 61 CGGGCCGTGG ATTCTACTCA ACTTTGTCGG CTTGAGAAAG ACCTGGGATC CTGGGTATTA 121 AAAAGAAGAT CTATTTATTT AGAGATCTGTTCTATTGTGA TCTCTTATTA GGATCGCACT 181 GCCCTGTGGA TAACAAGGAT CCGGCTTTTA AGATCAACAA CCTGGAAAGG ATCATTAACT 241 GTGAATGATC GGTGATCCTG GACCGTATAA GCTGGGATCA GAATGAGGGG TTATACACAA 301 CTCAAAAACT GAACAACAGT TGTTCTTTGG ATAACTACCG GTTGATCCAA GCTTCCTGAC 361 AGAGTTATCC ACAGTAGATC GCACGATCTG TATACTTATT TGAGTAAATT AACCCACGAT
Proteins needed for initiation at oriC #1 • DnaA • Only used at initiation • Mutations cause a slow-stop phenotype • Binds to the 4 copies of 9 bp repeats • Further cooperative binding brings in 20 to 40 DnaA monomers • Melts the DNA at the 3- 13 bp repeats
Proteins needed for initiation at oriC #2 • DnaB • ATP-dependent helicase • Displaces DnaA and unwinds DNA further to form replication forks • “Activates” primase, apparently by stablizing a secondary structure in single-stranded DNA • DnaC • Is in complex with DnaB before loading onto template
Proteins needed for initiation at oriC #3 • DnaG primase • Gyrase • SSB • All but DnaA are also used in elongation
Positions of ter sequences in E. coli Replication fork 2
Termination of replication: DNA sites and proteins needed • DNA sites: ter sequences, 23 bp • terD and terA block progress of counter-clockwise fork, allow clockwise fork to pass • terC and terB block progress of clockwise fork, allow counter- clockwise fork to pass • Protein: Tus • “ter utilization substance” • Binds to ter • Prevents helicase action from a specific replication fork
Control by methylation • GATC motifs are substrates for methylation by dam methylase. • Methylase transfers a methyl group from S-adenosylmethionine to N-6 of adenine in GATC. • Methylated GATC on BOTH strands: oriC will serve as an origin • Methylated GATC on ONLY one strand (hemimethylated): oriC is not active • Re-methylation is slow, delays use of oriC to start another round of replication.
Regulation of replication by methylation m m G A T C G A T C C T A G C T A G m m G A T C methylate replicate C T A G (lags m m G A T C G A T C behind replication) C T A G C T A G m m dam methylase Hemimethylated Fully methylated Fully methylated Will not replicate Will replicate Will replicate