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Where are bacteriophages found?. Source Phages counted Soil 10 4 /gm River 10 3 - 10 9 /ml Seawater 10 7 /ml Feces - human 10 9 /gm. Estimated # of phages in biosphere: 10 30. Lytic life cycle. human DNA. 50 kb.
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Where are bacteriophages found? SourcePhages counted Soil 104/gm River 103 - 109/ml Seawater 107/ml Feces - human 109/gm Estimated # of phages in biosphere: 1030
human DNA 50 kb
Bacteriophage vectors are useful for cloning larger DNA fragments Bacteriophage Vectors
8kb 15kb 7kb 15kb 8kb 7kb 15kb 8kb 7kb concatenati 7kb 7kb
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In vitro packaging Uso di batteri lisogeni contenenti Differenti mutazioni nei geni strutturali I batteri sono cresciuti a densità voluta E poi indotti al ciclo litico
Genoteca Insieme di segmenti di DNA o cDNA inseriti in vettori fagici o plasmidici
40-45 kb DNA umano sequenze cos Antibiotico R sequenze cos
Filamentous phages RF Replicative Form