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Thin Layer Chromatography

Thin Layer Chromatography. bsapp.com. Preparing a Sample Space. Cut a sheet of paper so it may hang free within the intended container Mark a sample line ½ inch to an inch from the bottom. bsapp.com. Place your samples on the sheets. bsapp.com. Starting the Sample.

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Thin Layer Chromatography

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  1. Thin Layer Chromatography bsapp.com

  2. Preparing a Sample Space • Cut a sheet of paper so it may hang free within the intended container • Mark a sample line ½ inch to an inch from the bottom bsapp.com

  3. Place your samples on the sheets bsapp.com

  4. Starting the Sample • Place the sheet in a container with a solvent • The bottom of the paper should be submerged into the solvent, but not above the samples bsapp.com

  5. Allow your samples to run at least ½ way up the sample space bsapp.com

  6. bsapp.com

  7. Allow your samples to dry bsapp.com

  8. Matching Samples • Note component colors • Calculate Rf values bsapp.com

  9. Calculating Rf Values • Rf value is a ratio of distance a component sample moves to the distance the solvent moves • This number is usually expressed as a decimal bsapp.com

  10. Solvent finish 10 cm Calculating Rf Values • 8 cm • 4 cm Rf = 4/10 = 0.4 Rf = 8/10 = 0.8 • Starting point bsapp.com

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