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Standard Deviation of the residuals. Standard Deviation of the slope. Standard Deviation of the intercept. Factors involved in determining uncertainty of calculated concentration. Number of replicates Number of calibration points Std. dev. of residuals
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Standard Deviation of the residuals Standard Deviation of the slope Standard Deviation of the intercept
Factors involved in determining uncertainty of calculated concentration • Number of replicates • Number of calibration points • Std. dev. of residuals • Location of unknown’s response on calibration curve
Standard Deviation of the residuals Standard Deviation of the slope Standard Deviation of the intercept Standard Deviation of the x value determined by calibration curve
Accuracy • Analyze standard reference material • Compare results from 2 or more analytical methods • Analyze blank sample spiked with known addition of analyte • Make standard additions of analyte to unknown