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Biocontrol

Biocontrol. Justin Kim. Need. Knowledge Base. Harsh Pal Bais (2000). Jorge M. Vivanco (2002). Biocontrol. R. Charudattan (2006). R. Charudattan (2006). Biocontrol Agent. (Elizabeth and Emmert, 1999). Bacillus Subtilis. Kung-Ta Lee (2001). Kung-Ta Lee (1993). Literature Review.

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Biocontrol

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  1. Biocontrol Justin Kim

  2. Need

  3. Knowledge Base Harsh Pal Bais (2000) Jorge M. Vivanco (2002)

  4. Biocontrol R. Charudattan (2006) R. Charudattan (2006)

  5. Biocontrol Agent • (Elizabeth and Emmert, 1999).

  6. Bacillus Subtilis Kung-Ta Lee (2001) Kung-Ta Lee (1993)

  7. Literature Review Fall, Ray (2004)

  8. Disease Ross Ballard (2008) Jason Brantner (2004)

  9. Methodology Flow Chart Arabidopsis/Soybean N= 120 Control Group- Seeds grown in normal environment N=30 V3- Plant inoculated with Pseudomonas Putida from fusarium N=30 V1- plant grown with the fusarium N=30 V2- Plant inoculated with bacillus subtilis from the fusarium N=30 Mortality of the plants are compared Mean +- SD Two way Anova

  10. Do-Ability • Biocontrols available from Online Carolina Catalog

  11. Purpose- To find biocontrol agents that works better against certain diseases Hypothesis- Alternative- Bacillus subtilis worked better against the disease Alternative- Pseudomonas putida worked better against the disease

  12. Budget Plan

  13. Bibliography • Baker et al., "Inhibitory effect of Bacillus subtilis on Uromyces phaseoli and on development of rust pustules on bean leaves" Phytopathology 73:1148-1152 (1983). • Cavaglieri L., Orlando J., Rodríguez M.I. - Biocontrol of Bacillus subtilis against Fusarium verticillioides in vitro and at the maize root level- Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36 Km, 601, (5800) Río Cuarto, Córdoba, Argentina • Elizabeth AB, Emmert HJ. Biocontrol of plant disease: A (Gram-)positive perspective. FEMS Microbiol Lett. 1999;171:1–9 • Estruch et al., "Transgenic plants: An emerging approach to pest control" Nature Biotechnology 15:137-141 (1997). • G. Heidi . Elnaga, Abo - Bacillus subtilis as a Biocontrol Agent for Controlling Sugar Beet Damping-off Disease -Egypt. J. Phytopathol., Vol. 34, No.1, pp. 51-59 (2006) • Olsson J, Bőrjesson T, Lundstedt T, Schnürer J. Detection and quantification of ochratoxin A and deoxynivalenol in barley grains by GC-MS and electronic nose. Int J Food Microbiol. 2002;72:203–214. • Onji Y, Aoki Y, Tani N, Umebayashi K, Kitada Y, Dohi Y. Direct analysis of several Fusarium mycotoxins in cereals by capillary gas chromatography-mass spectrometry. J Chromatogr A. 1998;815:59–65. • Placinta CM, D’Mello JPF, Macdonald AMC. A view of worldwide contamination of cereal grains and animal feed with Fusarium mycotoxins. Anim Feed Sci Tech. 1999;78:21–37. • Pelczar, Michael. Pelczar, Rita- “plant disease”- Encyclopædia Britannica. 2009. Encyclopædia Britannica Online • Singh et al., "Bacillus subtilis as a control agent against fungal pathogens of citrus fruit" Trans. Br. Mycol. Soc. 83(3):487-490 (1984). • Williams & Wilkins- Bergey's Manual of Sytematic Bacteriology, , vol. 2, pp. 1123-1124, 1126, 1133 (1986). • Xin Zhang,1 Bing-xin Zhang,†‡1 Zhen Zhang,2 Wei-feng Shen,2 Ching-hong Yang,3 Jing-quan Yu,4 and Yu-hua Zhao- Survival of the biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116 on the spikes of barley in the field- Department of Plant Protection, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China • Yamada et al., "Biological activity of antifungal substances produced by Bacillus subtilis" J. Pesticide Sci. 15(1):95-96 (1990).

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