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RECOMBINANT DNA TECHNOLOGY

RECOMBINANT DNA TECHNOLOGY. PART 2. Topics. Genomic libraries Id a specific clone or sequence within a library Transgenic plants Transgenic animals Timely topics. Genomic library contains all of the organism’s genome. Probe design. Bacterial clones and finding desired clone.

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RECOMBINANT DNA TECHNOLOGY

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  1. RECOMBINANT DNA TECHNOLOGY PART 2

  2. Topics • Genomic libraries • Id a specific clone or sequence within a library • Transgenic plants • Transgenic animals • Timely topics

  3. Genomic library contains all of the organism’s genome Probe design

  4. Bacterial clones and finding desired clone Probe design

  5. A Final about vectors : There are many types  • Small vectors • Large vectors • Expression vectors • Viral vectors

  6. Transgenic Mice

  7. transgene Non-homologous recombination LinearDNA http://spine.rutgers.edu/cellbio/assets/flash/transgen.htm

  8. Knockout Mice

  9. G418 gancyclovir Homozygous knockout http://www.bio.davidson.edu/courses/genomics/method/homolrecomb.htm Phenotype of the knockout mouse gives information on protein function

  10. Applications of DNA technology • Agriculture • Drug design • Genetic testing • Gene therapy

  11. Genetic Modified Plants: “GM” Tobacco with luciferase gene from firefly Transgenic plant

  12. Tumor Inducing Plasmid

  13. Model for making genetically modified (GM) plants

  14. GM crops • Bt toxin genes (Bacillus thuringensis)

  15. Bt Toxin Cry toxins and cry genes: damages gut of larvae

  16. GM Plants • Roundup  ready plants: herbicide resistant • Herbicide known as: Glyphosate

  17. Glyphosate • Enzyme inhibitor • Prevents amino acid synthesis • Prevents weed growth • Environmental concerns

  18. Antisense Technology • 5´C A U G   3´     mRNA3´   G U A C   5´     Antisense RNA

  19. Anti-Sense Technology &FLAVR SAVRTM Tomato • Anti-sense DNA technology inhibits polygalacturonase (PG)

  20. The Flavr-Savr Tomato • Ripens on the vine • Preserves firmness and flavor • Uses anti-sense technology

  21. Cloning of a whole organism: Reproductive Cloning

  22. SHEEP CELLS 54 27 Somatic Cell Germ Cell For ex. Mammary cell ovum

  23. How to process begins: 54 27 54 discarded Somatic Cell Germ Cell For ex. Mammary cell ovum

  24. Now the germ cell contains 54 chromosomes! • The germ is stimulated “electrically”. • Cell division in some instances begins. • Individual is identical to host “DNA” donor. • Aging affects? “Was Dolly a sheep in lamb’s clothing?”

  25. Somatic Gene Therapy

  26. Successes • SCIDS (Severe Combined Immunodeficiency Disease) • Head and neck cancers

  27. But also failures and controversy

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