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Sample Name: JW04-011 Location: Arkashin

PROGRESS IN THE ISOLATION OF ANAREOBIC THERMOPHILIC CROTONATE & METHANOL-UTILIZING MICROORGANISMS Arkashin. Sample Name: JW04-011 Location: Arkashin. The third Balch agar-shake roll-tube dilution series prepared for multiple cultures at: 1) 60°C, ph 6, and methanol as substrate

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Sample Name: JW04-011 Location: Arkashin

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  1. PROGRESS IN THE ISOLATION OF ANAREOBIC THERMOPHILIC CROTONATE & METHANOL-UTILIZING MICROORGANISMS Arkashin Sample Name: JW04-011 Location: Arkashin The third Balch agar-shake roll-tube dilution series prepared for multiple cultures at: 1) 60°C, ph 6, and methanol as substrate 2) 60°C, ph 6, and crotonate as substrate 16S rRNA analysis to follow shortly! then characterization; pH/temp optima, substrate range, G+C content, etc.

  2. PROGRESS IN THE ISOLATION OF ANAREOBIC THERMOPHILIC CROTONATE & METHANOL-UTILIZING MICROORGANISMS North Thermal Field Sample Name: JW04-013 Location: Yellow Pool 10m South of CZ2-NTF The third Balch agar-shake roll-tube dilution series prepared for multiple cultures at: 1) 60°C, ph 6, and methanol as substrate 2) 60°C, ph 6, and crotonate as substrate 16S rRNA analysis to follow shortly! then characterization; pH/temp optima, substrate range, G+C content, etc. Colonies observed in the first Balch agar-shake roll-tube dilution series with multiple cultures at: 3) 60°C, ph 9, and crotonate as substrate Subsequent Balch agar-shake roll-tube dilution rounds will be preformed

  3. PROGRESS IN THE ISOLATION OF ANAREOBIC THERMOPHILIC CROTONATE & METHANOL-UTILIZING MICROORGANISMS Jen’s Pool Sample Name: JW04-006 Location: Jen’s Pool, Vent 2 Growth observed in enrichment subcultures at: 1) 74°C, ph 6, and methanol as substrate 2) 80°C, ph 6, and methanol as substrate A means of isolation will be employed (Gelrite, rather than agar may be needed for the Balch roll-tubes)

  4. PROGRESS IN THE ISOLATION OF ANAREOBIC THERMOPHILIC CROTONATE & METHANOL-UTILIZING MICROORGANISMS Jen’s Pool Sample Name: JW04-007 Location: Small Pool 2m East of Jen’s Pool, Vent 2 The third Balch agar-shake roll-tube dilution series prepared for multiple cultures at: 1) 60°C, ph 6, and methanol as substrate 2) 60°C, ph 6, and crotonate as substrate 16S rRNA analysis to follow shortly! then characterization; pH/temp optima, substrate range, G+C content, etc. Colonies observed in the first Balch agar-shake roll-tube dilution series with multiple cultures at: 3) 60°C, ph 9, and crotonate as substrate Subsequent Balch agar-shake roll-tube dilution rounds will be preformed

  5. PROGRESS IN THE ISOLATION OF ANAREOBIC THERMOPHILIC CROTONATE & METHANOL-UTILIZING MICROORGANISMS Jen’s Pool Sample Name: JW04-008 Location: Jen’s Pool, Vent 1 Growth observed in enrichment subcultures at: 1) 74°C, ph 6, and methanol as substrate 2) 80°C, ph 6, and methanol as substrate A means of isolation will be employed (Gelrite, rather than agar may be needed for the Balch roll-tubes)

  6. PROGRESS IN THE ISOLATION OF ANAREOBIC THERMOPHILIC CROTONATE & METHANOL-UTILIZING MICROORGANISMSCentral/K4 Sample Name: JW04-010 Location: Blue-Grey Pool System (W. CTF) The third Balch agar-shake roll-tube dilution series prepared for multiple cultures at: 1) 60°C, ph 6, and crotonate as substrate 16S rRNA analysis to follow shortly! then characterization; pH/temp optima, substrate range, G+C content, etc. Colonies observed in the first Balch agar-shake roll-tube dilution series at: 2) 60°C, ph 9, and methanol as substrate Subsequent Balch agar-shake roll-tube dilution rounds will be preformed

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