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(a). ( b ). PDC1. 3.5. WT. 3. npt1∆. 2.5. 2. NAD/NADH ratio. Relative expression. 1.5. 1. 0.5. 0. log. 3. 6. 9. 12. log. 3. 6. 9. 12. hrs post log phase. hrs post log phase. ( c ). ( d ). [0- 1093]. 500 bp. Input. WT. [0- 666]. sir2∆. hst1∆. Sir2. x. sum1∆.
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(a) (b) PDC1 3.5 WT 3 npt1∆ 2.5 2 NAD/NADH ratio Relative expression 1.5 1 0.5 0 log 3 6 9 12 log 3 6 9 12 hrs post log phase hrs post log phase (c) (d) [0- 1093] 500 bp Input WT [0- 666] sir2∆ hst1∆ Sir2 x sum1∆ [0- 500] Glucose (mg/ml) Hst1 [0- 565] Sum1 0 3 6 9 12 15 18 PDC5 hours (e) (f) PDC1 PDC5 WT WT sir2∆ sir2∆ D sir2∆+vec hst1∆ sir2∆+WT npt1∆ sir2∆+H364Y D Relative expression Relative expression log 3 6 9 12 log 3 6 9 12 hrs post log phase hrs post log phase
