1 / 27

NMR Spectroscopy

NMR Spectroscopy. Two-dimensional NMR. http://www.univ-lille1.fr/lcom/RMN2D/resum2D_us/index_us.htm. 1D NMR. Pulse Sequence. Spectrum (EtOH). Four periods in 2D experiment. Preparation spin system relaxes and then excited by r.f.

xiang
Download Presentation

NMR Spectroscopy

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. NMR Spectroscopy Two-dimensional NMR http://www.univ-lille1.fr/lcom/RMN2D/resum2D_us/index_us.htm

  2. 1D NMR Pulse Sequence Spectrum (EtOH)

  3. Four periods in 2D experiment Preparation spin system relaxes and then excited by r.f. Evolution (t1) chemical shifts & spin-spin couplings evolve. This is the timedomain which is incremented during a 2D experiment Mixing r.f. pulses are applied and create observable transverse magnetization Detection (t2) observable transverse magnetization is recorded. It is usually labeled with t2

  4. 2D NMR • Primary 2D matrix consists a series of FIDs. • A set of 1D NMR spectra is obtained by Fourier transformation with respect to t2 • The signals of each transformation may differ in amplitude and phase. A second Fourier transformation with respect to t1 yields the final 2D matrix with frequency axes F1 and F2 1. 2. 3.

  5. 2D NMR Pulse Sequence Spectrum

  6. 2D NMR

  7. 2D NMR

  8. 2D NMR

  9. 2D J-resolved COSY 45.COSY 90.COSY DQF.TOCSY. COSY L.R.TOCSY Relayed COSY. (with one homonuclear relayed). NOESY.ROESY.

  10. 2D J-resolved spectroscopy

  11. H,H- COSY Experiment

  12. COSY & TOCSY

  13. TOCSY of strychnine

  14. NOESY of strychnine

  15. C,H Correlation by Polarization Transfer (HETCOR)

  16. HMQC The HMQC experiment provides correlation between protons and their attached heteronuclei through the heteronuclear scalar coupling. HMBC The HMBC experiment suppresses correlations via 1JC,H while 2J, 3J can be conserved. HSQC The HSQC experiment is in fact a double INEPT experiment. This experiment correlates protons with their directly attached heteronuclei. (G. Bodenhausen and D. J. Ruben, Chem. Phys. Lett., 69, 185 (1980)

  17. Basic HMQC Experiment F2 displays the doublets with the spin coupling constant 1J(C-H) in addition to the H,H spin coupling.

  18. HMQC with Decoupling Only singlets are displayed in F2 . These signals are further split by H,H spin couplings.

  19. Basic HMBC Experiment Cross signals caused by 1J of C-6 and C-3 are still observable but those arising from 3J and 2J are predominant.

  20. Basic HSQC Experiment

  21. COSY

  22. DQF-COSY

  23. HMQC

  24. HMBC

  25. HSQC

More Related