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International Hands-on workshop on genotoxiciy monitoring bioassays for environmental pollution

International Hands-on workshop on genotoxiciy monitoring bioassays for environmental pollution. University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland Directed by

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International Hands-on workshop on genotoxiciy monitoring bioassays for environmental pollution

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  1. International Hands-on workshop on genotoxiciy monitoring bioassays for environmental pollution University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland Directed by Prof. JolantaMaluszynska, Department of Plant Anatomy and Cytology, University of Silesia, Katowice, Poland Prof. Siegfried Knasmueller, Institute for Cancer Research, The Medical University of Vienna, Vienna, Austria Prof. Michael Plewa, University of Illinois, Champaign-Urbana, IL, USA

  2. International workshop on genotoxicity monitoring bioassays for environmental pollution Group photo of the participants

  3. Prof. Knasmueller (left) and Prof. Maluszynska (right) discuss workshop plan

  4. Prof. JolantaMaluszynska giving instructions at the workshop, demonstrating Fluorescent in situ hybridization (FISH) assay (photomicrograph was not available)

  5. Instructor of the workshop giving a lecture to the participants.

  6. Prof. Michael Plewa of the University of Illinois demonstrating the slide preparation for Comet test for clastogens

  7. Photomicrograph of a comet tail as the result of DNA damage by clastogens

  8. Mr. Bernhard Majer of the medical University of Vienna prepares Allium roots and Tradescantia cuttings for chromosome aberration and micronucleus assays .

  9. Tradescantia plants bearing young inflorescences provide the test material for both Tradescantia- Stamen-Hair (Trad-SHM) and Tradescantia-Micronucleus (Trad-MCN) assays

  10. Photomicrograph of Sister-Chromatid-Exchange (SCE) in Tradescantia root tip cell Photomicrograph of Sister-Chromatid-Exchange (SCE) in Tradescantia root tip cell cell.

  11. Dissection of flower buds in preparation of Trad-MCN slides from the meiotic microsporocytes

  12. Preparing stamen hairs of Tradescantia flower under a dissecting microscope for scoring frequencies of pink mutation events in the Trad-SHM assay.

  13. MCN MCN Photomicrograph showing micronuclei (MCN) among the resting stage root tip cells in the subsequent generation of treated nuclei

  14. MCN MCN MCN Photomicrograph of the tetrad stage of microsporocyte of Tradescantia showing micronuclei (MCN) among the synchronized tetrads in Trad-MCN assay

  15. Scoring pink mutation events under the dissecting microscope in the Tad-SHM assay

  16. Tradescantia flowers ready for scoring pink mutation events after a 16- minute refrigeration in the Trad-SHM assay

  17. blue pink blue pink Photomicrograph of orderly aligned stamen hairs showing 3 pink mutation events in this field of view in the Trad-SHM assay

  18. Collecting data for frequencies of mutation and micronueli in treated and control groups of Trad-SHM and Trad-MCN assays

  19. DATA analysis using computer software and print out for report write up

  20. After a day’s hard work, participants took a tour around town

  21. Relaxing and shopping in the Mall in the city of Katowice, Poland

  22. Let’s have some coffee and snack

  23. A walk outside to have some fresh air

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