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XbaI

Park et al., Supplementary file 1. XbaI. XbaI. Ag mutator pol3 gene (4.9kb). BamHI. MCS. ori. G418 R. BamHI. YCpG418/ pold exo- (12.3kb). MCS. Amp R. CEN4. ARS1. Supplementary file 1. Plasmid map of YCpG418/ pold exo-

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XbaI

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  1. Park et al., Supplementary file 1 XbaI XbaI Ag mutator pol3 gene (4.9kb) BamHI MCS ori G418R BamHI YCpG418/poldexo- (12.3kb) MCS Amp R CEN4 ARS1 Supplementary file 1. Plasmid map of YCpG418/poldexo- A 12.3 kb YCpG418/poldexo- vector was used for transformation. This vector was constructed from YCplac111 vector (6.1 kb) which has a low copy numbers vector, with deletion of LEU2 molecule (between AatII-EcoRV sites, 1.2 kb); and insertion of G418 cassette (2.5 kb) in MCS fragment at BamHI site. AshbyaPOL3 gene (4.9 kb) fragment was inserted at XbaI site of MCS .

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