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MOLECULAR GENETICS. Majury Arumai Puneet Kaur Mandy So Robert Wallis. OVERALL EXPECTATIONS. BIG IDEAS. C1. Analyse some of the social, ethical, and legal issues associated with genetic research and biotechnology. DNA contains all the genetic information for any living organism.
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MOLECULAR GENETICS Majury Arumai Puneet Kaur Mandy So Robert Wallis
OVERALL EXPECTATIONS BIG IDEAS C1. Analyse some of the social, ethical, and legal issues associated with genetic research and biotechnology DNA contains all the genetic information for any living organism How can we use our knowledge of molecular genetics to understand the scientific, social, legal, and ethical issues associated with the topics presented in this unit? C2. Investigate, through laboratory activities, the structures of cell components and their roles in processes that occur within the cell Proteins control a wide variety of cellular processes C3. Demonstrate an understanding of concepts related to molecular genetics, and how genetic modifications is applied in industry and agriculture Genetic research and biotechnology have social, legal, and ethical implications • How does the concept of central dogma guide the understanding of molecular genetics? • How do advances in biotechnology expand our knowledge base? • How do we balance scientific progress in biotechnology with bioethical concerns?
D3.1 explain the current model of DNA replication, and describe the different repair mechanisms that can correct mistakes in DNA sequencing D2.2 analyse simulated strand of DNA to determine the genetic code and base pairing of DNA D3.7 describe, on the basis of research, some historical scientific contributions that have advanced our understanding of molecular genetics D2.4 investigate and analyse the cell components involved in protein synthesis using appropriate lab equipment and techniques/computer simulation D1.1 analyse on the basis of research, some of the social, ethical, and legal implications of biotechnology D3.3 explain the steps involved in protein synthesis and how genetic expression is controlled in prokaryotes and eukaryotes by regulatory proteins D2.1 use appropriate terminology related to molecular genetics D3.2 compare the structures and functions of RNA and DNA, and explain their roles in protein synthesis D1.2 analyse, on the basis of research, some key aspects o Canadian regulations regarding biotechnology D3.4 explain how mutagens can cause mutations by changing the genetic material in cells. D2.3 conduct an investigation to extract DNA from a plant or animal specimen D3.5 describe examples of genetic modification, and explain how it is applied in industry and agriculture D3.6 describe the functions of some of the cell components used in biotechnology
DNA EXTRACTION LAB
EXPECTATIONS • A1.1 – formulate hypotheses to focus inquiries or research • A1.5 – conduct inquiries using appropriate materials and equipment to collect observations • A1.8 – confirm/refute hypotheses • A1.10 – draw conclusions based on inquiry results • A1.11 – communicate plans, procedures & results • D2.3 – conduct an investigation to extract DNA from a specimen of plant or animal
What do we need? BANANA PESTLE & MORTAR DETERGENT SALT CONTACTS SOLUTION RUBBING ALCOHOL STRAINER
STEP 1: BREAK THE CELL WALL
STEP 2: GET RID OF SOLIDS
STEP 3: BREAK THE MEMBRANES
STEP 4: PROTEIN BREAKDOWN PROTEASES
PRECIPITATE THE DNA STEP 5: 70-90%
Lab Groups 1 2 3 Laura Amy P Chris Wong Alessia Susan Meryl Sally Tyler Paul Jane Melanie Chris Walsh 5 4 6 Sheena Karen Philip Katarina Ruth Gabe Jenny Brad Alicia Amy Z.
Central Dogma replication DNA transcription RNA translation Protein
Basepairing A A T U C C DNA G G RNA
DNA T T U U A A A G G G C C C mRNA tRNA Glu
Letters of the alphabet U U C E tRNA Glu
ANSWERS 1) THE CENTRAL DOGMA IS ON THE TEST 2) PHIL DO WE HAVE THE TWENTY FIFTH OFF
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