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TRAP

Dr. Paul Gollnick. Regulation of the tryptophan genes in Bacillus subtilis. TRAP. t rp R NA-binding A ttenuation P rotein. - tryptophan. + tryptophan. TRAP does not. TRAP is Activated. Bind RNA. to Bind RNA. trp genes are Expressed. trp genes are Repressed.

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TRAP

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  1. Dr. Paul Gollnick Regulation of the tryptophan genes in Bacillus subtilis TRAP trpRNA-binding Attenuation Protein -tryptophan +tryptophan TRAP doesnot TRAP isActivated Bind RNA toBind RNA trp genes areExpressed trp genes areRepressed

  2. Attenuation control of the B. subtilis trp operon

  3. The Structure of TRAP Antson et al. Nature 374; 693-700, 1995

  4. The Structure of TRAP bound to RNA Antson et al. Nature 401; 235-242 1999

  5. Crystal structure of TRAP complexed with (GAGAU)11 Antson et al. Nature 401; 235-242 1999

  6. Attenuation control of the B. subtilis trp operon

  7. TRAP Binds to RNA by a 5’ to 3’ Directional Mechanism 3’ GAG GAG GAG GAG GAG GAG GAG 5’ 5’ GAG GAG GAG GAG 3’

  8. TRAP binding induces RNA cleavage

  9. Attenuation control of the B. subtilis trp operon

  10. TRAP-RNA TRAP-RNA RNA RNA Anti-TRAP prevents TRAP from binding RNA AT added prior to RNA AT KD = 10.1 μM RNA added prior to AT AT Yanling Chen

  11. Crystal structure of Anti-TRAP Mikhail Shevtsov and Alfred Antson

  12. Nonsense Mediated Decay (NMD) Several Proteins involved in degrading mRNA Contain PIN Domain

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