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Do HIV+ Rapid Progressors Show More Divergence than Non-Progressors?

Do HIV+ Rapid Progressors Show More Divergence than Non-Progressors? . Karen Klyczek, Alix Darden, Susan Godfrey, Karl Beres, and Mark Bergland. Hypothesis.

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Do HIV+ Rapid Progressors Show More Divergence than Non-Progressors?

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  1. Do HIV+ Rapid Progressors Show More Divergence than Non-Progressors? Karen Klyczek, Alix Darden, Susan Godfrey, Karl Beres, and Mark Bergland

  2. Hypothesis Rapidly progressing HIV+ subjects will show more divergence in their HIV sequences between their first and last visits, compared to non-progressors.

  3. Methods Study population: a cohort of IV drug users in Baltimore -six rapid progressors - < 200 CD4 T cells/microliter (subjects 1, 3, 4, 10, 11, 15) -three non-progressors - > 650 CD4 T cells/microliter (subjects 2, 12, 13) Generation of phylogenetic trees: 285 bp regions of the HIV-1 env gene was amplified for all subjects. Data from Markam et al. (1998) analyzed using ClustalW (Biology Workbench)

  4. Table 1. Subject parameters for HIV study.

  5. Progressors

  6. Progressors Subject 3

  7. Progressors Subject #4 Visit #1

  8. Progressors Subject 10

  9. Progressors Subject 11

  10. Progressors

  11. Nonprogressors Subject 2

  12. Nonprogressors

  13. Nonprogressors Subject 13

  14. Conclusions and Discussion Overall, rapid progressors exhibited more divergence in their HIV sequences than non-progressors. In rapid progressors, sequences from clones isolated on their first visit tended to cluster separately from clones isolated on their last visit. In two of three non-progressors, there was less separation between sequences obtained from the first and last visits. More data from non-progressors are needed to confirm these differences. Reference: Markham, R.B. et al. (1998) P.N.A.S. USA 95:12568.

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