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  1. * * * * * * * NS NS Figure S1. Validation of Hoxa9/ Meis1 ChIP-seq results by ChIP-QPCR. ChIP was done using anit-HA antibodies on a HA-Hoxa9/FLAG-Meis1 cell line and a non epitope-tagged cell line derived from transformed murine bone marrow. Real-Time PCR quantification of the ChIP samples was quantified relative to inputs using Taqman primer/probes. Binding is detected at the Hoxa9/ Meis1 binding sites for HA-Hoxa9(A) and FLAG-Meis1(B) in the HA-Hoxa9/FLAG-Meis1 cell line whereas only background signal is detected in the non-epitoped tagged control cell line. A standard t-test was performed to examine statistical significance of Hoxa9/Meis1 binding in HA-Hoxa9/FLAG-Meis1 compared to untagged Hoxa9/Meis1 cell line and randomly selected genomic control regions. ([*] P< 0.0001; [NS] Not significant).

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