t--/----./)rt

1. t t'*r/' ) t--/----./)rt v 7 \*_ - 2010 Journal Biotechnology 9(53), 9042-9045, December' pp. 27 Vol. of African at http://www.academicjournals.org/AJB Available online Journals Academic O2010 ISSN1684-5315 PaPer Full LengthResearch variation sampleof lranian in Detection genetic of marker cattleby usingmicrosatellite proofed Holstein Mohammad Reza Movahedint, Cyrus Amirinia2*, Alireza Nosharyl and S. Ahmad Mirhadi2 lDeoartment Animal lran. Mehr Shahr, Karaj, AzadUniversity, lslamic Branch, Karaj ' 2Department Science, of lran. lnstitute lran,Karaj, of Research Animal Science of Biotechnology, Holsteincattle (Bos taurus\by using This study describesgeneticvariation samplesof individuals "; -""ianfollowedby DNAextraction. panelof "-:::tTt." A were taken markerslsemen microsatellite was used for evaluation 13 loci in 68 Holsteinproofedbulls. Meanvalue for allele of 13 microsatellites DNA markers pertocus detectedis 6.615,rangingfrom 10 (sPS115) 4 (ETH3). the microsatellite All to high polymorphismanA aisptayeda relativelyhigh level of geneticvariationas estimatedby showed rangedfrom 1.000(8M2113, TGLA122' Estimatedheterozygosities allelic diveisity ahd heterozygosity. with meanvalueof 0.946. and INRAO23) 0.633(SPS113) to TGLA227 S-p-SrrS, MGTG4B, ETI-{3, TGLA126, information polymorphism All the loci showeddeviaiionfrom Hardy-Weinbergequilibrium(p < 0.001), index content(plc) calculated each markerexceeded and the meanvalueof Shannoninlormation 0.6 for heterozygousity be usefulfor the development can resultsshowing was estimated be 1.606. Obtained to cattle' of breedingstrategies geneticimprovement lranianHolstein in for DNA. Holstein cattle, microsatellite Bostaurus,lranian loci, polymorphism, variability, Key words: Genetic INTRODUCTION cattlemicro- Thereare morethana thousand al., 2005). Livestockindustryrequiresthe development very of {rom (Barendse al., 1994; et markers choose to needs satellite cattlepopulation fulfillits commercial to standard The usefulness microsatellite of Kappes al., 1997). et by selectionpracticesin breedingprograms. Genetic has markers the estimation genetic diversity been of for allowsfarmersto develop found in population diversity (Buchanan al..1994. et studies documented numerous in in new characteristics response changes environ- to in et Maudet Schmid al., 1999: et Saitbekova al., 1999; et (Maudet al.' et ment.diseases, marketconditions or al..2002). of 2002). The extensiveselectionand multiplication This studywas conducted determine levelsof the to decreasein the animalscause a significant superior DNA poly- microsatellite variation examining genetic by needed the improvement econo- of for genetic variation proofed Holstein lranian among individual 68 morphisms mic traitsand breeds(Machado al., 2003).Genetic et to according bullsand 13 microsatellite werechosen loci the genetic data also can be used to monitor diversity panelrecommended FAO (2004), by the microsatellites structure populations detectchanges the fre- in and of mar- and alsoto determine efficiency microsatellite of the whichmakes programs, due to breeding quency genes of kersin estimation levels genetic diversity. of of diversity farm of it possible preserve the biological to et Martin-Burriel (Moazami-Goudarzi 1997; et al., animals Maudet al.,2002). et al.,1999; AND METHODS MATERIALS have provedto be usefulpolymorphic Microsatellites (Guolizhou et markers theanalysis genetic diversity of for Sampling were testedand proofed individuals Semensamples 68 progeny- of from Animal BreedingCenter o{ lran (Kar4, lran) and collected within liquid nilrogen.GenomicDNA delivered the laboratory to usingprotocols previously as -Corresponding author. E-mail: amirinia@asri'ir Tel/fax: were extracted from semensamples (1990). and Kuhenlein described Zadworny by 0 443001 +98261