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E-cad. Snai2. FOSL1. ACTB. -. -. miR-138 mimic control mimic anti-miR-138 LNA control LNA FOSL1 siRNA control siRNA. -. -. -. -. -. -. +. +. -. -. -. -. -. -. -. -. +. +. -. -. -. -. -. -. -. -. -. +. -. -. -. -. -. -. -. -. -. +. -. -. -. -. -.
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E-cad Snai2 FOSL1 ACTB - - miR-138 mimic control mimic anti-miR-138 LNA control LNA FOSL1 siRNA control siRNA - - - - - - + + - - - - - - - - + + - - - - - - - - - + - - - - - - - - - + - - - - - - - - + + - - - - - - - - + + UM1 686Tu 1386Ln Supplementary Figure 4: The effect of miR-138 and FOSL1 on the expression of Snai2 and E-cadherin in HNSCC cells. UM1 and 1386Ln cells were treated with either miR-138 mimic or control mimic, or FOSL1 specific siRNA or control siRNA, and 686Tu cells were treated with either anti-miR-138 LNA or negative control LNA. The expression of FOSL1, Sani2 and E-cadherin was examined by western blots.