1 / 11

ThermoTRP channels expression in the broodpatch of junglefowl during egg incubation

ThermoTRP channels expression in the broodpatch of junglefowl during egg incubation. The regulation of egg temperature requires the transfer of heat from the brood patch skin and regulation of broodpatch blood flow The broodpatch needs:

yitta
Download Presentation

ThermoTRP channels expression in the broodpatch of junglefowl during egg incubation

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. ThermoTRP channels expression in the broodpatch of junglefowl during egg incubation • The regulation of egg temperature requires the transfer of heat from the brood patch skin and regulation of broodpatch blood flow • The broodpatch needs: • The presence of thermo receptors namely thermoTRPs • An appropriate vasomotor response and morphological change • We tried to detect : • The presence of thermoTRP channels (V1, V3, V4, A1 and M8) in different junglefowl tissues • Changes of brood patch: Regulation in TRP channels? • Morphological changes?

  2. M8 in all checked tissues (Heart, Kidney, Liver, DRG, Blood, WBC and SKIN) • V1 in all except Blood • V3 in all except Blood and WBC • A1 in None except Liver and DRG

  3. M8 in all checked tissues (Heart, Kidney, Liver, DRG, Blood, WBC and SKIN • V1 in all except Blood • V3 in all except Blood and WBC • A1 in None except Liver and DRG Only Genomic fragments for V4

  4. M8 in all checked tissues (Heart, Kidney, Liver, DRG, Blood, WBC and SKIN • V1 in all except Blood • V3 in all except Blood and WBC • A1 in None except Liver and DRG In the skin Only Genomic fragments for V4

  5. * *

  6. * * *

  7. * * * * *

  8. * * * • No change in V1 & V3 expression in qPCR! • M8? * *

  9. thermoTRP’s expression in junglefowl ‘s tissues • TRPA1 not in the skin. Why? • TRPV4 mRNA was not found in any tissue. Why? • Not expressed? • Alternative splicing? • Brood patch morphological changes • Epidermal increase, AVA’s frequency amplification, AVA’s area growth • TRP channels regulation in Epidermis? • Or AVAs? Or in the earlier stages?

  10. Although we can see considerable morphological changes in the skin of broodpatch the tested thermoTRP channel’s expression did not show significant change • TRP channels are so specific to some parts of skin? • TRP channels regulation is only in AVAs or epidermis? Or in first stages of incubation? • V4 a mystery! • Thank you!

More Related