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BLES + 30 mol% d7-chol

ToF-SIMS negative ion images of two BLES (bovine lipid extract surfactant) LB films containing 0 mol% (A, C) and 30 mol% (B, D) cholesterol-d7. M255 corresponds to the palmitate group, M2 to deuterium present in the tail of cholesterol-d7.

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BLES + 30 mol% d7-chol

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  1. ToF-SIMS negative ion images of two BLES (bovine lipid extract surfactant) LB films containing 0 mol% (A, C) and 30 mol% (B, D) cholesterol-d7. M255 corresponds to the palmitate group, M2 to deuterium present in the tail of cholesterol-d7. Images E, F, G, H were obtained by subjecting A, B, C, D, respectively to a smoothing function available as part of the IonTof software, and show more clearly the location of the palmitate group and deuterium. Images are 50 x 50 mm2, 256 x 256 pixels with 1 shot/pixel. BLES + 30 mol% d7-chol BLES A B M255 D C M2 F E M255 processed G H M2 processed This set of images is published in the Biophysical Journal.  The goal of that work was to determine the function of cholesterol in an animal extracted surfactant (BLES).  TOF-SIMS was used to determine the location of cholesterol in an LB-film of BLES. Courtesy of Eleonora Keating, Univ. of Western Ontario

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