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Tg (hsp70:mcherry; CMV:naos3’UTR:GFP)

Tg (hsp70:mcherry; CMV:naos3’UTR:GFP). 8 hrs. 16 hrs. Express EGFP in germ cell. 24 hrs. Tg (dkk1b-2Ki1). Express EGFP in pharyngeal arch and otic vesicle. TgIBStrap1. Day 1 . Day 2 . Expressed in lateral line. Tg (gnrh3-GFP-LacZ).

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Tg (hsp70:mcherry; CMV:naos3’UTR:GFP)

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  1. Tg(hsp70:mcherry; CMV:naos3’UTR:GFP) 8 hrs 16 hrs Express EGFP in germ cell 24 hrs

  2. Tg(dkk1b-2Ki1) Express EGFP in pharyngeal arch and oticvesicle.

  3. TgIBStrap1 Day 1 Day 2 Expressed in lateral line

  4. Tg(gnrh3-GFP-LacZ) Expression and migration of GFP- and LacZ-expressing cells. A, B, F, G, ventral view; C-E, dorsal view. A, C, F, G, GFP cells; D, E, LacZ-expressing cells. A, GFP expression at the olfactory placode (OP). B-D, GFP or LacZ cells begin to migrate into telencephalon (Tel) at 3-8 d (days). E, lacZ cells and axons were detected in brain at 16dpf. F-G, At 1 month (m), fluorescent axonal extensions are detected at OB, Tel and POA. Red arrows indicate cell bodies, yellow arrows indicate axons. POA, preoptic area.

  5. Tg-dkk1b-1k ,1kE1, 2k, 4k C D E 2k 2k:GFP; 3d 2k:GFP; 2d 2k:GFP; 1d G H F 1k 1k:GFP; 1d 1k:GFP; 3d 1k:GFP; 2d I J K 1kE1 1kE1:GFP; 3d 1kE1:GFP; 1d 1kE1:GFP; 2d L M N 4k 4k:GFP; 3d 4k:GFP; 1d 4k:GFP; 2d Expressed in pharyngeal arches, otic vesicle, whole body(4k)

  6. Tg(zpopdc1:GFP)

  7. Tg(J11:EGFP)

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