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Iron-Reducing Bacteria. Hopkins Microbiology Course Presented by Ying Lei 22 July 2010. Reduction Process. Fe 3+ only – ferrihydrite (FeOOH · 0.4H 2 O) – orange/red solid Fe 3+ /Fe 2+ – magnetite (Fe 3 O 4 ) – black, ferromagnetic solid
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Iron-Reducing Bacteria Hopkins Microbiology Course Presented by Ying Lei 22 July 2010
Reduction Process • Fe3+ only– ferrihydrite (FeOOH · 0.4H2O)– orange/red solid • Fe3+/Fe2+– magnetite (Fe3O4)– black, ferromagnetic solid • Fe2+ only– ferrous iron– black, non-magnetic solid
Redox Tower CH3CH(OH)COO- + 12 FeOOH + 4 H2O 3 HCO3- + 12 Fe2+ + 22 OH- lactate C0 iron (III) Fe3+ CO2 C4+ iron (II) Fe2+ Canfield et al, Aquatic Geomicrobiology (2005)
Enrichment Medium Adjust to pH 7.2 in Pfennig bottles and incubate under anoxic conditions. Hopkins Microbiology Course (2010)
Sample Collection Canfield et al, Aquatic Geomicrobiology (2005)
Successful Enrichments Marcus
Thank You All Instructors and Students Chris Marcus Blanca
Iron Solubility OzCoasts (www.ozcoasts.org.au)