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mal3. 

Grallert et al. Supplementary Figure 1. 0. 0. 0. 0. 0. 0. 0. 0. 10. 10. 10. 10. 10. 10. 10. 10. 20. 20. 20. 20. 20. 20. 20. 20. 30. 30. 30. 30. 30. 30. 30. 30. 40. 40. 40. 40. 40. 40. 40. 40. 50. 50. 50. 50. 50. 50. 50. 50. 60. 60. 60. 60. 60.

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mal3. 

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  1. Grallert et al. Supplementary Figure 1 0 0 0 0 0 0 0 0 10 10 10 10 10 10 10 10 20 20 20 20 20 20 20 20 30 30 30 30 30 30 30 30 40 40 40 40 40 40 40 40 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 70 70 70 70 70 70 70 70 80 80 80 80 80 80 80 80 90 90 90 90 90 90 90 90 100 100 100 100 100 100 100 100 110 110 110 110 110 110 110 110 120 120 120 120 120 120 120 120 130 130 130 130 130 130 130 130 140 140 140 140 140 140 140 140 peg1.1 mal3. tip1. peg1.1 dlc1. dlc2. peg1.1 mal3. peg1.1 tip1. mal3. tip1. peg1.1 dhc1. mal3. tip1. 5m

  2. Grallert et al. Supplementary Figure 2 tip1. nmt1GFPatb2 20M thiamine 2M thiamine 5m

  3. Grallert et al. Supplementary Figure 3 Relative a-tubulin level GFPatb2 nda2 atb2 Cdc2 tip1., 36C peg1.1, 25C peg1.1, 36C tip1., 25C wild type, 25C wild type, 36C nmt81GFPatb2, 25C nmt81GFPatb2, 36C nmt81GFPatb2, 25C nmt81GFPatb2, 36C peg1.1 mal3., 25C peg1.1 mal3., 36C peg1.1 nmt81GFPatb2, 25C peg1.1 nmt81GFPatb2, 36C mal3. nmt81GFPatb2, 25C tip1. nmt81GFPatb2, 25C tip1. nmt81GFPatb2, 36C peg1.1 nmt81GFPatb2, 25C peg1.1 nmt81GFPatb2, 36C mal3. nmt81GFPatb2, 36C nmt81GFPatb2 + ppeg1.c, 25C tip1. peg1.1 nmt81GFPatb2, 25C tip1. peg1.1 nmt81GFPatb2, 36C peg1.1 mal3. nmt81GFPatb2, 25C peg1.1 mal3. nmt81GFPatb2, 36C nmt81GFPatb2 + p ppeg1.c, 36C mal3. nmt81GFPatb2 + pDB20, 25C peg1.1 nmt81GFPatb2 + p ppeg1.c, 25C peg1.1 nmt81GFPatb2 + p ppeg1.c, 36C mal3. nmt81GFPatb2 + pDB20,, 36C

  4. Grallert et al. Supplementary Figure 4 wild type tip1. mal3. peg1.1 tip1. peg1.1 mal3. peg1.1 dlc1. dlc2. tip1. mal3. dhc1. tip1. mal3. peg1.1 dlc1. dlc2. peg1.1 5m dhc1. peg1.1

  5. Grallert et al. Supplementary Figure 5 Merged GFP signal Lectin marker to identify tea1.pkGFP cells Brightfield to identify all cells in field 5m tea1.pkGFP + peg1.1 mal3. tea1.pkGFP tea1.pkGFP tea1.pkGFP + mal3.tea1.pkGFP tea1.pkGFP + peg1.1 tea1.pkGFP

  6. 0 min 1 min 2 min 3 min 4 min 5 min wild type peg1.1 0 min 1 min 2 min 3 min 4 min peg1.1 wild type Grallert et al. Supplementary Figure 6 A Log phase culture > ice/salt bath 20 mins > re-warm to 36°C B 16 h Nitrogen starvation > ice/salt bath 20 mins > re-warm to 36°C 10 m

  7. Grallert et al. Supplementary Figure 7 Before MT reaches cell tip As MT curls around cell tip After MT reaches cell tip wild type peg1.1 dhc1. peg1.1 dhc1.

  8. Grallert et al. Supplementary Table 1

  9. Grallert et al. Supplementary Table 2

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