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Chapter 6 – Microbial Growth

Chapter 6 – Microbial Growth. Growth of Bacterial Cultures. Physical Requirements. Culture Media. Measurement of Growth. Chemical Requirements. $100. $100. $100. $ 100. $100. $200. $200. $200. $200. $200. $300. $300. $300. $300. $300. $400. $400. $400. $400. $400. $500.

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Chapter 6 – Microbial Growth

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  1. Chapter 6 – Microbial Growth Growth of Bacterial Cultures Physical Requirements Culture Media Measurement of Growth Chemical Requirements $100 $100 $100 $100 $100 $200 $200 $200 $200 $200 $300 $300 $300 $300 $300 $400 $400 $400 $400 $400 $500 $500 $500 $500 $500 FINAL ROUND

  2. Topic 1: Physical Requirements$100 Question • The optimum pH for most bacteria is • a. 1 • b. 5 • c. 7 • d. 9 ANSWER BACK TO GAME

  3. Topic 1:Physical Requirements$100 Answer • The optimum pH for most bacteria is • a. 1 • b. 5 • c. 7 (correct answer) • d. 9 BACK TO GAME

  4. Topic 1: Physical Requirements$200 Question • A psychrophile grows best at • a. 37°C • b. 0°C • c. 25°C • d. 15°C ANSWER BACK TO GAME

  5. Topic 1: Physical Requirements$200 Answer • A psychrophile grows best at • a. 37°C • b. 0°C (correct answer) • c. 25°C • d. 15°C BACK TO GAME

  6. Topic 1:Physical Requirements$300 Question • Organisms that require high salt concentrations for growth are called • a. Halophiles • b. Extreme halophiles • c. Facultative halophiles • d. Microhalophiles ANSWER BACK TO GAME

  7. Topic 1:Physical Requirements $300 Answer • Organisms that require high salt concentrations for growth are called • a. Halophiles • b. Extreme halophiles (correct answer) • c. Facultative halophiles • d. Microhalophiles BACK TO GAME

  8. Topic 1: Physical Requirements$400 Question • The energy source utilized by producers in the hydrothermal vents on the ocean floor is • a. Light • b. Sulfur • c. Organic molecules • d. Carbon dioxide ANSWER BACK TO GAME

  9. Topic 1:Physical Requirements$400 Answer • The energy source utilized by producers in the hydrothermal vents on the ocean floor is • a. Light • b. Sulfur (correct answer) • c. Organic molecules • d. Carbon dioxide BACK TO GAME

  10. Topic 1: Physical Requirements$500 Question • In food preservation, salts and sugars are used because they create a • a. Lower pH • b. Hypertonic environment • c. Hypotonic environment • d. Lower osmotic pressure ANSWER BACK TO GAME

  11. Topic 1: Physical Requirements$500 Answer • In food preservation, salts and sugars are used because they create a • a. Lower pH • b. Hypertonic environment (correct answer) • c. Hypotonic environment • d. Lower osmotic pressure BACK TO GAME

  12. Topic 2: Chemical Requirements$100 Question • Chemoautotrophs and photoautotrophs derive their carbon from • a. Carbon dioxide • b. Proteins • c. Lipids • d. Carbohydrates ANSWER BACK TO GAME

  13. Topic 2: Chemical Requirements$100 Answer • Chemoautotrophs and photoautotrophs derive their carbon from • a. Carbon dioxide (correct answer) • b. Proteins • c. Lipids • d. Carbohydrates BACK TO GAME

  14. Topic 2:Chemical Requirements$200 Question • Some microorganisms use gaseous nitrogen directly from the atmosphere in a process called • a. Chemosynthesis • b. Photosynthesis • c. Nitrogen fixation • d. Nitrogen synthesis ANSWER BACK TO GAME

  15. Topic 2:Chemical Requirements$200 Answer • Some microorganisms use gaseous nitrogen directly from the atmosphere in a process called • a. Chemosynthesis • b. Photosynthesis • c. Nitrogen fixation (correct answer) • d. Nitrogen synthesis BACK TO GAME

  16. Topic 2: Chemical Requirements$300 Question • An organism that uses oxygen but can • grow without oxygen is called a(n) • a. Obligate aerobe • b. Facultative aerobe • c. Aerobe • d. Aerotolerant anaerobe ANSWER BACK TO GAME

  17. Topic 2: Chemical Requirements$300 Answer • An organism that uses oxygen but can • grow without oxygen is called a(n) • a. Obligate aerobe • b. Facultative aerobe (correct answer) • c. Aerobe • d. Aerotolerant anaerobe BACK TO GAME

  18. Topic 2: Chemical Requirements$400 Question • This organism is killed by atmospheric • oxygen: • a. Aerobe • b. Obligate aerobe • c. Obligate anaerobe • d. Aerotolerant anaerobe ANSWER BACK TO GAME

  19. Topic 2: Chemical Requirements$400 Answer • This organism is killed by atmospheric oxygen: • a. Aerobe • b. Obligate aerobe • c. Obligate anaerobe (correct answer) • d. Aerotolerant anaerobe BACK TO GAME

  20. Topic 2:Chemical Requirements$500 Question • Biofilms reside in a matrix made up primarily of • a. Lipids • b. Hydrogel • c. Proteins • d. Polysaccharides ANSWER BACK TO GAME

  21. Topic 2:Chemical Requirements$500 Answer • Biofilms reside in a matrix made up primarily of • a. Lipids • b. Hydrogel • c. Proteins • d. Polysaccharides (correct answer) BACK TO GAME

  22. Topic 3:Growth of Bacterial Cultures$100 Question • Generation time can best be defined as • a. The length of time it takes for lag phase • b. The duration of log phase • c. The length of time it takes for a cell to divide • d. The minimum rate of doubling ANSWER BACK TO GAME

  23. Topic 3:Growth of Bacterial Cultures$100 Answer • Generation time can best be defined as • a. The length of time it takes for lag phase • b. The duration of log phase • c. The length of time it takes for a cell to divide (correct answer) • d. The minimum rate of doubling BACK TO GAME

  24. Topic 3: Growth of Bacterial Cultures$200 Question • To cultivate anaerobic bacteria, one must use special media called • a. Reducing media • b. Oxidizing media • c. Differential media • d. Selective media ANSWER BACK TO GAME

  25. Topic 3:Growth of Bacterial Cultures$200 Answer • To cultivate anaerobic bacteria, one must use special media called • a. Reducing media (correct answer) • b. Oxidizing media • c. Differential media • d. Selective media BACK TO GAME

  26. Topic 3: Growth of Bacterial Cultures$300 Question • Capnophiles must be grown in high concentrations of • a. Oxygen • b. Salt • c. Glucose • d. Carbon dioxide ANSWER BACK TO GAME

  27. Topic 3:Growth of Bacterial Cultures$300 Answer • Capnophiles must be grown in high concentrations of • a. Oxygen • b. Salt • c. Glucose • d. Carbon dioxide (correct answer) BACK TO GAME

  28. Topic 3:Growth of Bacterial Cultures$400 Question • The culture medium that is designed to increase numbers of desired microbes to detectable levels is called • a. Complex • b. Selective • c. Enrichment • d. Differential ANSWER BACK TO GAME

  29. Topic 3: Growth of Bacterial Cultures$400 Answer • The culture medium that is designed to increase numbers of desired microbes to detectable levels is called • a. Complex • b. Selective • c. Enrichment (correct answer) • d. Differential BACK TO GAME

  30. Topic 3: Growth of Bacterial Cultures$500 Question • The culture medium that is designed to suppress unwanted microbes while encouraging desired microbes is called • a. Complex • b. Selective • c. Reducing • d. Differential ANSWER BACK TO GAME

  31. Topic 3:Growth of Bacterial Cultures$500 Answer • The culture medium that is designed to suppress unwanted microbes while encouraging desired microbes is called • a. Complex • b. Selective (correct answer) • c. Reducing • d. Differential BACK TO GAME

  32. Topic 4:Culture Media$100 Question • To neutralize acids as they are produced in a culture, what should be added to the culture medium? • a. Buffers • b. Salts • c. Sugars • d. PABA ANSWER BACK TO GAME

  33. Topic 4:Culture Media$100 Answer • To neutralize acids as they are produced in a culture, what should be added to the culture medium? • a. Buffers (correct answer) • b. Salts • c. Sugars • d. PABA BACK TO GAME

  34. Topic 4: Culture Media$200 Question • The microbes that grow and multiply in or on a culture medium are referred to as a(n) • a. Culture • b. Inoculum • c. Specimen • d. Medium ANSWER BACK TO GAME

  35. Topic 4:Culture Media$200 Answer • The microbes that grow and multiply in or on a culture medium are referred to as a(n) • a. Culture (correct answer) • b. Inoculum • c. Specimen • d. Medium BACK TO GAME

  36. Topic 4: Culture Media$300 Question • What can be added to a medium when it is desirable to grow bacteria on a solid medium? • a. Glucose • b. Magnesium sulfate • c. Potassium phosphate • d. Agar ANSWER BACK TO GAME

  37. Topic 4:Culture Media$300 Answer • What can be added to a medium when it is desirable to grow bacteria on a solid medium? • a. Glucose • b. Magnesium sulfate • c. Potassium phosphate • d. Agar (correct answer) BACK TO GAME

  38. Topic 4:Culture Media$400 Question • A culture medium consisting of agar, human blood, and beef heart is a • a. Complex medium • b. Selective medium • c. Differential medium • d. Chemically defined medium ANSWER BACK TO GAME

  39. Topic 4:Culture Media$400 Answer • A culture medium consisting of agar, human blood, and beef heart is a • a. Complex medium (correct answer) • b. Selective medium • c. Differential medium • d. Chemically defined medium BACK TO GAME

  40. Topic 4: Culture Media$500 Question • The source of nutrients in nutrient agar is • a. Agar • b. Peptone and sodium chloride • c. Peptone and beef extract • d. Nutrient ANSWER BACK TO GAME

  41. Topic 4:Culture Media$500 Answer • The source of nutrients in nutrient agar is • a. Agar • b. Peptone and sodium chloride • c. Peptone and beef extract (correct answer) • d. Nutrient BACK TO GAME

  42. Topic 5: Measurement of Growth$100 Question • A spectrophotometer can be used to measure • a. Metabolic activity • b. Turbidity • c. Dry weight • d. Pathogenicity ANSWER BACK TO GAME

  43. Topic 5: Measurement of Growth$100 Answer • A spectrophotometer can be used to measure • a. Metabolic activity • b. Turbidity (correct answer) • c. Dry weight • d. Pathogenicity BACK TO GAME

  44. Topic 5:Measurement of Growth$200 Question • Because of time considerations, which method is usually used to count the number of bacteria in milk? • a. The direct microscope count • method • b. The pour plate method • c. The spread plate method • d. The most probable number method ANSWER BACK TO GAME

  45. Topic 5:Measurement of Growth$200 Answer • Because of time considerations, which method is usually used to count the number of bacteria in milk? • a. The direct microscope count • method (correct answer) • b. The pour plate method • c. The spread plate method • d. The most probable number method BACK TO GAME

  46. Topic 5: Measurement of Growth$300 Question • Which method is utilized to ensure that plates containing 25 to 250 colonies are counted? • a. The most probable number method • b. Serial dilution • c. Filtration • d. Spread plate method ANSWER BACK TO GAME

  47. Topic 5: Measurement of Growth$300 Answer • Which method is utilized to ensure that plates containing 25 to 250 colonies are counted? • a. The most probable number method • b. Serial dilution (correct answer) • c. Filtration • d. Spread plate method BACK TO GAME

  48. Topic 5: Measurement of Growth$400 Question • Microorganisms are particularly sensitive to adverse conditions during the • a. Lag phase • b. Death phase • c. Stationary phase • d. Log phase ANSWER BACK TO GAME

  49. Topic 5: Measurement of Growth$400 Answer • Microorganisms are particularly sensitive to adverse conditions during the • a. Lag phase • b. Death phase • c. Stationary phase • d. Log phase (correct answer) BACK TO GAME

  50. Topic 5: Measurement of Growth$500 Question • Which of the following metabolic activities can be used to measure microbial growth? • a. Carbon dioxide consumption • b. Oxygen consumption • c. Glucose consumption • d. Salt consumption ANSWER BACK TO GAME

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