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Tephritid Barcoding Initiative

Tephritid Barcoding Initiative. Tephritid Barcoding Initiative. CBoL obtained funding from Sloan Foundation to support a “Demonstrator System” Mid-size taxon, reasonable taxonomic knowledge Interested user community Scientists willing to commit to 2-year project

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Tephritid Barcoding Initiative

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  1. Tephritid Barcoding Initiative

  2. Tephritid Barcoding Initiative • CBoL obtained funding from Sloan Foundation to support a “Demonstrator System” • Mid-size taxon, reasonable taxonomic knowledge • Interested user community • Scientists willing to commit to 2-year project • CBoL approached the mosquito and fruit fly communities to submit proposals (support confirmed September 2006)

  3. Tephritid Barcoding Initiative • Family Tephritidae (>4,000 species, >350 economically important species) • Anastrepha, Bactrocera, Ceratitis, Dacus, Rhagoletis • Morphological keys of adults are available for many pests and congeners • Declining taxonomic expertise • Extensive museum collections

  4. Tephritid Barcoding Initiative • Generate barcode database for 2,000 species (10,000 individuals; use of museum specimens) • Train postdoctoral scholars in morphological and molecular approaches to tephritid systematics • Establish globally-available DNA repository • Develop protocols for queries to DNA barcode database in support of pest management, ecology and taxonomy

  5. Tephritid Barcoding Initiative • Statement of cooperation from >15 major museums • Funding estimate: US$1 million direct costs • Funding from Sloan Foundation, USDA-APHIS, Penn State, Belgian government • Collaboration several Plant Protection Agencies

  6. Tephritid Barcoding Initiative • Slow start: • Funding • Older museum specimens • Current state • Approx 500 specimens sequenced (177 taxa of which 82 EI) • 335 extractions ready for PCR (additional 50 taxa of which 45 EI) • Several acquisitions from museums • Most sequences come from taxa in EI genera: Bactrocera, Dacus, Ceratitis, Anastrepha, Rhagoletis • Large part of sequences come from taxa in species complexes • Aim end of 2007: at least half of EI taxa. • July 2008: all EI taxa

  7. Tephritid Barcoding Initiative • Where it works (African Bactrocera and Dacus) NJ tree K2P

  8. Tephritid Barcoding Initiative • … and where it doesn’t (Ceratitis FAR complex) NJ tree K2P fasciventris anonae rosa (cf. poster at this conference by Virgilio et al.)

  9. Tephritid Barcoding Initiative Contact: Bruce McPheron, Penn State, USA Chair, TBI Steering Committee bam10@psu.edu TBI Coordinators: Allen Norrbom, USDA, USA Marc De Meyer, RMCA, Belgium Steering Committee Members: Karen Armstrong, New Zealand Norman Barr, USA Amnon Freidberg, Israel Ho-Yeon Han, South Korea George Roderick, USA Ian White, UK Presentation Chair and Coordinators, in collaboration with: Karen Armstrong, Peter Kerr, Massimiliano Virgilio

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