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Daphnia Toxicology Experiment

Daphnia Toxicology Experiment. Bridges 2014. Daphnia. Freshwater planktonic crustaceans C yclic parthenogenesis Favorable conditions: Asexual reproduction of females Unfavorable conditions: Sexual reproduction Reproductive maturity: 3-5 days Fine mesh feeders Eat bacteria and algae

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Daphnia Toxicology Experiment

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  1. Daphnia Toxicology Experiment Bridges 2014

  2. Daphnia • Freshwater planktonic crustaceans • Cyclic parthenogenesis • Favorable conditions: Asexual reproduction of females • Unfavorable conditions: Sexual reproduction • Reproductive maturity: 3-5 days • Fine mesh feeders • Eat bacteria and algae • Swim with an erratic, jerking motion then hang motionless in the water • Many predators, including fish, shrimp and copepods

  3. Toxicity • The degree to which a substance can damage an organism or the environment • Toxicity is dose dependent • Too much water can be deadly • Just a little snake venom can be deadly • Many household products are toxic • This is an issue when they enter the environment or our drinking water!

  4. Examples Household Toxins • Drain cleaners • Oven cleaners • Laundry detergents • Floor or furniture polish • Paints • Pesticides • Disinfectants • Cleaning Products • Many others…

  5. Testing Toxicity with Daphnia • Pick a substance that you think will be toxic to Daphnia • Create serial dilution of substance • Think carefully about what dilutions to use • Should include: • Positive control (will kill) • Negative control (won’t kill) • Transfer live Daphnia to plate containing dilution of potentially toxic substance • Score dead/live Daphnia in each treatment daily

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