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DNA methylation analysis

DNA methylation analysis. Approach High -throughput bisulfite sequencing: Gill tissue Additional resources: RNA- seq data: g ill tissue (Zhang et al, 2012). g enomic DNA. DNA methylation analysis. Approach High -throughput bisulfite sequencing: G ill tissue Additional resources:

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DNA methylation analysis

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  1. DNA methylation analysis • Approach • High-throughput bisulfite sequencing: • Gill tissue • Additional resources: • RNA-seqdata: gill tissue (Zhang et al, 2012) genomic DNA

  2. DNA methylation analysis • Approach • High-throughput bisulfite sequencing: • Gill tissue • Additional resources: • RNA-seqdata: gill tissue (Zhang et al, 2012) genomic DNA

  3. 0bp 200,000bp CpG Using genomic intervals to summarize biological data genes exons ex %methylation 100% 0% (Galaxy Trackster)

  4. Mac: Using genomic intervals to summarize biological data

  5. Mac: Using genomic intervals to summarize biological data SQLShare

  6. Mac: Using genomic intervals to summarize biological data SQLShare bedtools

  7. Mac: Using genomic intervals to summarize biological data SQLShare bedtools Excel

  8. Mac: Using genomic intervals to summarize biological data SQLShare bedtools Excel R

  9. Tools • SQLShare– table manipulation, filtering, etc • Galaxy/IGV – genome browsing • iPlant – lots of genomic tools! • Evernote – notebook • R – analysis

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