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Explore how hypothetical proteins can be optimized using different fusion tags such as FLAG-tag, GPI signal peptide, and 6xHis-tag. This study investigates the impact of various recombinant protein modifications on expression and functionality.
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hypothetical protein recombinant protein 25 346 23 433 352 360 PFD1130w 362 aa 439 aa 25 282 289 376 23 297 PF14_0325 301 aa 376 aa 358 439 22 148 169 327 346 23 25 151 172 330 366 20 PFF0620c 371 aa 445 aa Secretion signal peptide Secretion signal peptide of bee-venom melittin FLAG-tag GPI attachment signal Transmembrane domain of mouse glycophorine S48/45 domain 6xHis-tag