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A. Amp r. (2464 bp). T3. T7. B. Expanded view of region within vector. LacZ. cos. cos. Total length including expanded region = 43kb Lambda left (to EcoRI) = ~19 kb Lambda right (from EcoRI) = ~24 kb. C. cos. cos. Total length including central stuffer = 43kb
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A Ampr (2464 bp) T3 T7 B Expanded view of region within vector LacZ cos cos Total length including expanded region = 43kb Lambda left (to EcoRI) = ~19 kb Lambda right (from EcoRI) = ~24 kb C cos cos Total length including central stuffer = 43kb The ends of the vector shown here are identical to those of vector B. They are just schematized differently. Vector Page XbaI→ EcoRI→ HindIII→ Not I→