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Crystal Structure of the Ribosome at 5.5 Å Resolution

Crystal Structure of the Ribosome at 5.5 Å Resolution. Marat M. Yusupov, 1 * Gulnara Zh. Yusupova, 1 * Albion Baucom, 1 Kate Lieberman, 1 Thomas N. Earnest, 2 J. H. D. Cate, 3 Harry F. Noller 1

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Crystal Structure of the Ribosome at 5.5 Å Resolution

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  1. Crystal Structure of the Ribosome at 5.5 Å Resolution • Marat M. Yusupov,1* Gulnara Zh. Yusupova,1* Albion Baucom,1 Kate Lieberman,1 Thomas N. Earnest,2 J. H. D. Cate,3 Harry F. Noller1 • 1 Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, CA 95064, USA. 2 Berkeley Center for Structural Biology, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. 3 Whitehead Institute, Cambridge, MA 01242, USA.

  2. Describe the three-dimensional structure of the 70S ribosome of the completed Thermus thermophilus containing mRNA and tRNA bound to the A and E site at 5.5 Å resulution and to the A site at 7 Å resolution. • Overrall structure of the 70s ribosome. • The densityof tRNA, bound to the P site of the 70S ribosome, at 5.5 Å resolution. • Views of the stucture of the T.thermophilus 70S ribosome. • Secondary and tertiary structure of 16S, 23S,and 5S RNA. • Differences between the conformations of 70Sribosomes and isolated subunits. • Stuctures of the intersubunit bridges. • tRNA-ribosome interactions. • Implications for the mechanism of translation.

  3. Electron density of tRNAMetf bound to the P site of the 70S ribosome, at 5.5 Å resolution

  4. Subunits of prokaryotic ribosomes(70S)

  5. A:30s核糖体背面观 • B：右面观（30S亚基左，50S右） • C：从50S亚基背面观 • D：左面观 • E：上面观（50S上） • F：50S界面观（interface，） • G：300S界面观（A旋转180度） • [灰色：23SRNA；淡绿色：5SRNA；兰绿色：16SRNA；紫红色：50S蛋白质。A：金黄色；P：桔黄色；E：红色 70S核糖体的结构

  6. Secondary and tertiary structures of 16S, 23S, and 5S rRNAs(A:16SRNA; B:23S, 5SRNA; C:Three-dimentional fold of 16s RNA; D: C:Three-dimentional fold of 23S and 5s RNA.)

  7. Conformational differences between rRNAs in 70S ribosomes and 30S and 50S subunits

  8. Intersubunit bridges

  9. Intersubunit bridges（continued）

  10. tRNA-ribosome interactions

  11. Two views of the A-tRNA anticodon stem loop bound to its codon in the 30S subunit A site

  12. Interaction of E-tRNA with the ribosome. • Secondary structures of 16S and 23S rRNA, showing molecular contacts with A-tRNA (gold), P-tRNA (orange), and E-tRNA (red)

  13. THANK YOU!BYE BYE!2002.09

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