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Magnetic Tweezer System Development

Magnetic Tweezer System Development. Probing mechanical properties across multiple scales. Jason Sherfey Senior BME, Vanderbilt University. Advisor: Dr. Franz Baudenbacher. Project Definition. Goal : to design, fabricate, test and miniaturize a microrheometer to quantify cell-cell adhesion

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Magnetic Tweezer System Development

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  1. Magnetic Tweezer System Development Probing mechanical properties across multiple scales Jason Sherfey Senior BME, Vanderbilt University Advisor: Dr. Franz Baudenbacher

  2. Project Definition • Goal: to design, fabricate, test and miniaturize a microrheometer to quantify cell-cell adhesion • Topics to Cover: Strategy, Reduction & Integration, Current Status, Project Schedule

  3. Strategy • Property measured: The cell-cell adhesion mechanics targeted consist of viscoelastic parameters of adhesion protein linker systems (static & dynamic responses) • Strategy: A magnetic tweezer – based device to perturb, image, and analyze linker system mechanics.

  4. Force 1 nN 3 2 T=0 s displacement [mm] 1 0 0 1 2 3 Time [s] F T=1.5 s Force displacement measurements on magnetic beads linked to the cell surface through E-Cadherin Fit to Mechanical Analog Extract Model parameter

  5. Accomplishments • Developed 1st generation MPT software • Fabricated magnetic tweezer and constructed 1st prototype • Established protocol to implement prototype • Validated strategy by experiment • Refined PT, MT, & protocol parameters to increase SNR & temporal resolution

  6. Current Status • On schedule • Effectiveness of strategy is confirmed • System parameters critical to the accuracy and reproducibility of acquired information have been identified and refined • The final phase of development involves reducing system size, mass, and complexity constrained by the desired critical system parameters

  7. Project Schedule • 1/29, Finish list of components that can be reduced, eliminated or subsume • 2/1, Determine relationship b/w listed mods and system parameters • 2/5, Finish list of methods to implement “allowable” mods (brainstorm, lit review, ..) • 2/8, Selected 2-3 best methods (minimize complexity & cost; max practicality) • 2/15, Have chosen 1 method to pursue; identified resources, costs, & practical approach • 2/22, Have started implementing modifications • 3/15, Have established a new prototype & begun refining • 3/29, Obtained final design prototype • 4/5 , Completing final reports, presentations, documentation

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