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Strawberry DNA Extraction

Strawberry DNA Extraction. DNA: Basics. D eoxyribo n ucleic a cid Genetic material Chain of molecules linked together DNA contains instructions for traits Wrapped tightly around “histones” Forms chromosomes Housed in nucleus. DNA: Basics. DNA: Structure. Double-helix (twisted ladder)

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Strawberry DNA Extraction

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  1. Strawberry DNA Extraction

  2. DNA: Basics • Deoxyribonucleic acid • Genetic material • Chain of molecules linked together • DNA contains instructions for traits • Wrapped tightly around “histones” • Forms chromosomes • Housed in nucleus

  3. DNA: Basics

  4. DNA: Structure • Double-helix (twisted ladder) • Sugar and phosphate backbone • Nitrogen bases • Subunit: “nucleotide”

  5. DNA: Replication • Special pairing between nitrogen bases • New strand is copy of old strand

  6. DNA Extraction: Step 1 • Break the cells open (using force)

  7. DNA Extraction: Step 2 • Dissolve the cell membrane and nuclear envelope (using detergent/soap) Current Protocols in Protein SciencePublished by John Wiley & Sons, Inc.

  8. DNA Extraction: Step 3 • Remove proteins that are bound to DNA (using salt to dissolve them)

  9. DNA Extraction: Step 4 • Precipitate DNA (with ethanol)

  10. DNA Extraction: Why? VS. Species identification

  11. DNA Extraction: Flow chart

  12. DNA Extraction: Flow chart

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