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p21/waf1 (mut). CDK2 + Cyc E. p21/waf1 (WT). P16/INK4A. Cyc D2. CDK2. CDK4. MW. 200 - 92 - 69 - 46 - 30 - 20 - 14 -. 1 2 3 4 5 6 7 8. A). B). CDK2 + CycE + p21/waf1 (WT, 100ng).

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  1. p21/waf1 (mut) CDK2 + Cyc E p21/waf1 (WT) P16/INK4A Cyc D2 CDK2 CDK4 MW 200 - 92 - 69 - 46 - 30 - 20 - 14 - . . . . . . . . . . . 1 2 3 4 5 6 7 8 A) B) CDK2 + CycE + p21/waf1 (WT, 100ng) CDK2 + CycE + p21/waf1 (mut, 200ng) CDK2 + CycE + p21/waf1 (WT, 200ng) CDK2 + CycE + p21/waf1 (mut, 100ng) CDK4 + CycD2 + p21/waf1 (mut) CDK4 + CycD2 + p21/waf1 (WT) CDK4 + CycD2 + p16/INK4A CDK4 + CycD2 CDK2 + CycE Cyc D2 CDK4 GST-Rb 1 2 3 4 5 6 7 8 9 10 11

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