Supplemental figures captions

1. Online supplementary material. Profound and persistent decrease of circulating dendritic cells is associated with ICU-acquired infection in patients with septic shock. Intensive care medicine Grimaldi D 1,2,3,4 *, Louis S 2,3,4 *, Pène F 1,2,3,4, Sirgo G 2,3,4, Rousseau C 2,3,4, Claessens YE 2,3,4,5,Vimeux L 2,3,4 Cariou A 1,2,Mira JP 1,2,3,4, Hosmalin A 2,3,4, Chiche JD 1,2,3,4 § 1 Medical Intensive Care Unit, Hôpital Cochin, AP-HP, 27 rue du Faubourg Saint Jacques 75014 Paris, France. 2 Université Paris-Descartes, 22 Rue Méchain 75014 Paris, France.3 Institut Cochin, INSERM U1016, 22 Rue Méchain 75014 Paris, France. 4 CNRS UMR 8104, 22 Rue Méchain 75014 Paris, France. 5 Emergency department, Hôpital Cochin, AP-HP, Paris, France § corresponding author: jean-daniel.chiche@cch.aphp.fr, tel: 33 1 58 41 25 01, fax: 33 1 58 41 25 05,

3. Supplemental figures captions Fig. S1: Dot plot of gating strategy. mDCs are CD45+, lineage-, HLA-DR & CD11c double positive cells, pDCs are CD45+, lineage-, HLA-DR & CD123 double positive. Corresponding isotype control for CD11c and CD123 (IgG1-APC and IgG1-PE respectively) were used. Typical example of healthy control (HC, upper panel) and patients with septic shock (SS, lower panel) are shown. Expression of HLA-DR is shown as fluorescence-activated cell sorter analysis and the corresponding MFI is indicated on the graph. Fig. S2: Evolution at day 1, 3 and 7 (D1, D3 D7) of HLA-DR MFI on circulating mDCs and pDCs in patients with septic shock (grey boxes) or non-septic shock (dashed boxes). Patients with less than 50 DCs/mL could not be analysed for HLA-DR expression. HLA-DR MFI decreased significantly in both groups and both DCs types (P=0.007 & P=0.0035 for mDCs and pDCs of septic shock group, P=0.02 & P=0.01). Fig. S3: Evolution of day 1, 3 and 7 (D1, D3 D7) mDCs and pDCs counts in patients from the non-septic shock group with (dashed boxes), or without (dotted boxes) ICU-acquired infection. Fig. S4: Evolution of individual mDC (upper panels) and pDC (lower panels) counts in patients with septic shock, which have developed (left panels) or not (right panels) ICU-acquired infections (IAI). Fig. S5: Evolution of individual HLA-DR expression on mDCs (upper panels) and pDCs (lower panels) in patients with septic shock, which have developed (left panels) or not (right panels) ICU-acquired infections (IAI).

4. Fig. S1 SSC PBMC PBMC CD45 CD45 lin- lin- HC HLA DR lin lin IgG1-APC IgG1-APC pDC pDC SSC HLA DR SS CD123-APC CD123-APC mDC IgG1-PE IgG1-PE mDC mDC CD11c-PE CD11c-PE MFI= 16.000 MFI= 12.000 mDC HLA DR HLA DR

5. Fig. S2 pDCs mDCs 25000 40000 20000 Septic shock group 30000 Non-septic shock group MFI 15000 20000 10000 10000 5000 0 0 N=43 N=29 N=36 N=17 N=21 N=8 N=35 N=26 N=30 N=17 N=17 N=7 D1 D3 D7 D1 D3 D7

6. Fig. S3 mDCs pDCs 18000 35000 Patients without IAI 14000 25000 Patients with IAI cells/ml 10000 15000 6000 5000 2000 0 0 N=25 N=4 N=14 N=3 N=6 N=2 N=25 N=4 N=14 N=3 N=6 N=2 D1 D3 D7 D1 D3 D7

7. mDC IAI+ mDC IAI- Fig. S4 cells/ml pDC IAI- pDC IAI+ cells/ml D1 D3 D7 D1 D3 D7

8. HLA-DR mDC IS+ HLA-DR mDC IS- Fig. S5 MFI HLA-DR pDC IS+ HLA-DR pDC IS- MFI D1 D3 D7 D1 D3 D7